Dr. Gilman-Sachs received her B.S. in 1962 from the University of Illinois at Urbana, M.S. in 1964 from the University of Illinois at Urbana, and her Ph.D. in Microbiology and Immunology in 1983 from the University of Illinois at Chicago, School of Health Sciences. She came to Rosalind Franklin University of Medicine and Science in 1985 and is Director of the Flow Cytometry Laboratory and Associate Director of the Clinical Immunology Laboratory. She is ABMLI Board Certified in Clinical Immunology.
Fifty percent of all recurrent spontaneous abortions (RSA) are unexplained and probably immune-related. My research involves the evaluation of pre- and post-immunization parameters in women who have received treatment to prevent RSA. One of the parameters studied in the past year is the ratio of TH1 vs TH2 cytokines. A successful pregnancy is supported by TH2 rather than TH1 cytokines at the maternal-fetal interface. Women with histories of recurrent spontaneous abortions may have a reversed ratio. Therefore, in this study the ratio of TH1/TH2 intracellular cytokines has been determined in women with two or more successful pregnancies and compared to women with histories of recurrent spontaneous abortions. These immune parameters are determined using peripheral blood lymphocytes and gating on CD4 and CD8 lymphocytes. The study indicates that there is indeed a difference between these two groups of women. The next set of experiments will be aimed at determining what treatments can reverse these ratios in RSA patients and allow a successful pregnancy to continue in women. Also, the ratios may be useful in monitoring and treating women with pregnancy loss.
Pregnancy loss is multifactorial and many immune related aspects are involved in it. Other factors being studied are activation markers on lymphocytes such as CD69 or CD25, natural killer cell (CD56+) cytokines, the relationship between KIR receptor genes of the female and HLA-C genes of the father in successful pregnancy, and last but not least the relationship between polymorphisms of cytokine promoters and pregnancy loss. By investigating the roles of these factors in successful or unsuccessful pregnancies, better treatment options may be utilized in women with recurrent spontaneous abortions.
Kwong C, Gilman-Sachs A, Beaman K. Tumor-associated a2 vacuolar ATPase acts as a key mediator of cancer-related inflammation by inducing pro-tumorigenic properties in monocytes. J Immunol. 1;186(3): 2011
Kim NY, Cho HJ, Kim HY, Yang KM, Ahn HK, Thornton S, Park JC, Beaman K, Gilman-Sachs A,
Kwak-Kim J. Thyroid Autoimmunity and its Association with Cellular and Humoral Immunity in Women with Reproductive Failures. Am J Reprod Immunol. 65:78-87, 2011
Lee S, Kim J, Jang B, Hur S, Jung U, Kil K, Na B, Lee M, Choi Y, Fukui A, Gilman-Sachs A
, Kwak-Kim JY. Fluctuation of peripheral blood T, B, and NK cells during a menstrual cycle of normal healthy women. J Immunol
. 2010 Jul 1;185(1):756-62. Epub 2010 Jun 7.
Yang KM, Ntrivalas E, Cho HJ, Kim NY, Beaman K, Gilman-Sachs A, Kwak-Kim J. Women with multiple implantation failures and recurrent pregnancy losses have increased peripheral blood T cell activation. Am J Reprod Immunol. 2010 May;63(5):370-8.
Ntrivalas E, Levine R, Kwong C, Gilman-Sachs A, Beaman K. The a2 isoform of vacuolar ATPase is a modulator of implantation and feto-maternal immune tolerance in early pregnancy. J Reprod Immunol. 2010 May;85(1):106-11
Lee SK, Na BJ, Kim JY, Hur SE, Lee M, Gilman-Sachs A, Kwak-Kim J. Determination of Clinical Cellular Immune Markers in Women with Recurrent Pregnancy Loss. Am J Reprod Immunol. 2013 May 9. doi: 10.1111/aji.12137.
Kwong C, Gilman-Sachs A, Beaman K. An independent endocytic pathway stimulates different monocyte subsets by the a2 N-terminus domain of vacuolar-ATPase. Oncoimmunology. 2013 Jan 1;2(1):e22978.
Jaiswal MK, Mallers TM, Larsen B, Kwak-Kim J, Chaouat G, Gilman-Sachs A, Beaman KD. V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy. Reproduction. 2012 May;143(5):713-25. doi: 10.1530/REP-12-0036. Epub 2012 Mar 27.
Beaman KD, Ntrivalas E, Mallers TM, Jaiswal MK, Kwak-Kim J, Gilman-Sachs A. Immune etiology of recurrent pregnancy loss and its diagnosis. Am J Reprod Immunol. 2012 Apr;67(4):311-8. doi: 10.1111/j.1600-0897.2012.01116.x. Epub 2012 Mar 1. Review.