The FACS Aria is a benchtop high speed cell sorter that can acquire up to 70,000 events/sec. It incorporates a fixed alignment cuvette flow cell with a fixed optical system and is armed with three lasers: (488nm argon blue , 633nm HeNe red,  and 405nm violet ). This permits multicolor detection of up to 9 colors along with FSC and SSC. The instrument can sort into 2 or 4 tubes, of various sizes, as well as into multiwell plates or slides. The FACSAria utililzes the latest version of PC-based FACSDiva software, which provides for enhanced sorting control and flexibility and data analysis.

FACS Aria Cell Sorter

LSRII is a three laser (488nm Coherent sapphire blue, 635nm HeNe and 405nm Coherent violet laser) benchtop system designed to support basic through advanced flow cytometric applications in up to 10 colors (12 parameters currently). The ability to reconfigure the optics in this machine provides extra flexibility in experimental design. This cytometer utilizes PC based FACS Diva acquisition and analysis software, although acquired data can be analyzed in CellQuest Pro also. ModFit Program, for DNA analysis is also available.

LSRII

FACS Calibur is a two laser (488nm argon ion and 635nm red diode laser) benchtop system of supporting basic cytometric applications in up to 4 colors. This cytometer utilizes Mac based CellQuest Pro acquisition and analysis software.

FACSCalibur

Contact

Department of Microbiology and Immunology

Robert Dickinson, Flow Cytometry Manager

Phone (847) 578-3000 x 3554

Room 2.218

E-mail: robert.dickinson@rosalindfranklin.edu