Patricio I. Meneses , Ph.D.
Assistant Professor
Room 2.351, Telephone (847) 578-8835
E-mail: patricio.meneses@rosalindfranklin.edu

Dr. Meneses received his B.S from the State University of New York at Stony Brook, Stony Brook, NY in 1990, and his Ph.D. at Cornell University Medical College, New York, NY, in Dr. Ken Bern's laboratory in 1999. He did his postdoctoral fellowship in the laboratory of Dr. Peter M. Howley in the Department of Pathology at Harvard Medical School. Dr. Meneses went on to the position of Senior Research Investigator at the University of Pennsylvania School of Medicine Department of Neurology, and Microbiology and Immunology. He joined the Department of Microbiology and Immunology at Rosalind Franklin University of Medicine and Science on January 1, 2006, as Assistant Professor.

Research
Cervical cancer is one of the leading causes of death in women worldwide. Papillomaviruses have been demonstrated to be the etiologic agent associated with over 99% of cervical cancers. Our interest lies in understanding the mechanism utilized by papillomavirus to establish a primary infection. The laboratory focuses on the role of the bovine papillomavirus type 1 (BPV-1), a prototype of papillomaviruses, minor capsid L2 during infection. This work has led to the discovery that BPV-1 traffics through the endoplasmic reticulum (ER) during infection in an L2 mediated event. L2 mediates this event by its interaction with the ER cellular protein syntaxin 18 that is involved in vesicle trafficking into and out of the ER. Our work was the first description of PV trafficking through the ER, a property shared by its papovavirus family members SV40 and murine polyomavirus. In addition to the role of L2 in intracellular trafficking, we have identified several novel interactions of L2 that suggest its role in 1) cellular senescence, 2) cell cycle regulation, and 3) regulation of the innate immune response. These are potential novel properties for a capsid protein. We have also identified a region of L2 that is highly conserved in all PV genotypes, as well as in other envelope and nonenveloped proteins involved in viral entry, whose mutation prevents infection. This region is being explored as a potential anti-viral target. The studies of the entry and intracellular trafficking mechanism used by papillomavirus described in these studies may be relevant to multiple non-enveloped and enveloped viruses. In addition PVs can be a very useful tool that can lead to a better understanding of the dynamic mechanism of basic cellular endocytosis, intracellular trafficking and sorting.

Selected Publications

Dabydeen SA, Meneses PI. The role of NH4CI and cysteine proteases in Human Papillomavirus type 16 infection Virology Journal 2009, 6:109.

Young A, Gentzch M, Abban CY, Jia Y, Meneses PI, Bridges RJ, Breadbury NA. Dynasore inhibits removal of wild-type and rF508 CFTR from the plasma membrane. IN PRESS Bioch Jou. Epub 2009, May 14th.

Laniosz, V., Dabydeen, S.A., Havens, M. A. & Meneses, P. I. (2009) Human Papillomavirus Type 16 Infection of Human Keratinocytes Requires Clathrin, Caveolin-1, and is Brefeldin A-Sensitive. J. Virol., JVI.00576-09. Epub 2009, June 3rd.

Holthusen KA, Gonzalez AM, Meneses PI. "Guanine exchange factor Vav2: a novel potential target for the development of drugs effective in the prevention of papillomavirus infection and disease. IN PRESS Am J Ther., Epub 2009, May 15th.

Laniosz V, Holthusen KA, Meneses PI. “Bovine papillomavirus type 1: from clathrin to caveolin.” J Virol., 2008 Jul;82 (13):6288-98. Epub, 2008, Apr 16.

Cynthia Y Abban, Neil A Bradbury, Patricio I. Meneses. HPV16 and BPV1 infection can be blocked by the dynamin inhibitor dynasore. American Journal of Therapeutics. 2008. Jul-Aug; 15 (4):304-311.

Masanao Murakami 1Y, Patricio I. Meneses 1, 2Y, Jason Knight1, Ke Lan1, Rajeev Kaul1, Subhash C. Verma1 and Erle S. Robertson 1*. Nm23-H1 modulates the activity of the guanine exchange factor Dbl-1. International Journal of Cancer, 2008. Aug. 1; 123 (3): 500-510. Y - equal contributors

Masanao Murakami, Patricio I. Meneses, Ke Lan and Erle S. Robertson. “The Suppressor of Metastasis Nm23-H1 Interacts with the Cdc42 Rho Family Member and the Pleckstrin Homology Domain of Oncoprotein Dbl-1 to Suppress Cell Migration”. Cancer Biology and Therapy. 2008. Vol 7; (5): 677-688. Cover Photograph.

Laniosz V, Nguyen KC, Meneses PI. Bovine papillomavirus type 1 infection is mediated by SNARE syntaxin 18”. J Virol. 2007 Jul;81(14):7435-48. Epub 2007, May 2.

Le, T.T., J.H. Levan and P.I. Meneses. "High-performance and grid computing for study and research in infectious diseases", Scientific conference on infectious disease and tropical medicine, pp 111-127, 2006.

Meneses, P.I., and E.S. Robertson. "Papillomavirus biology and therapeutic approaches," Gene. Ther. Biol., 9:217-228, 2005.

Bossis, I., R.B.S. Roden, R. Gambhira, R. Yang, M. Tagaya, P.M. Howley and P.I. Meneses. "Interaction of tSNARE Syntaxin 18 with the papillomavirus minor capsid protein mediates infection," J. Virol., 79:6723-6731, 2005.

Dalmau, J., F. Graus, A. Villarejo, J.B. Posner, D. Blumenthal, B. Thiessen, A. Saiz, P. Meneses and M.R. Rosenfeld. "Clinical analysis of anti-Ma2 associated encephalitis," Brain, 127(Pt 8):1831-44, 2004 Aug.

Berns, K.I., P. Meneses, R. Duvoisin, P. Laipis and W. Hauswirth. "Use of recombinant angiostatin to prevent retinal neovascularization," Trans. Am. Clin. Climatol Assoc., 112:68-75, 2001.

Meneses, P.I., K.A. Hajjar, K.I. Berns and R.M. Duvoisin. "Recombinant angiostatin prevents retinal neovascularization in a murine proliferative retinopathy model," Gene Ther., 8:646-648, 2001.

Meneses, P., K.I. Berns and E. Winocour."DNA sequence motifs which direct adeno-associated virus site-specific Integration in a model system," J. Virol., 74:6213-6216, 2000.

Meneses, P.I., L.E. Abrey, K.A. Hajjar, S.H. Gultekin, R.M. Duvoisin, K.I. Berns and M.R. Rosenfeld. "Simplified production of a recombinant human angiostatin derivative thatsuppresses intracebral glial tumor growth," Clin. Cancer Res., 5:3689-3694, 1999.

Rosenfeld, M.R., I. Bergman, L. Schramm, J.A. Griffin, M.G. Kaplitt and P.I. Meneses. "Adeno-associated viral vector gene transfer into leptomeningeal xenografts," J. Neuro-oncol., 34:139-144, 1997.

Rosenfeld, M.R., P. Meneses, J. Dalmau, M. Drobnjak, C. Cordon-Cardo and M.G. Kaplitt. "Gene transfer of wild-type p53 results in restoration of tumor suppressor function in a medulloblastoma cell line," Neurology, 45:1533-1539, 1995.

Meneses Lab: Kirsten A. Holthusen, Sarah A. Dabydeen, Dr. Valerie Laniosz, and Cynthia Y. Abban.

Page updated 07/21/09.