Email: physiology@rosalindfranklin.edu Fax: 847.578.3265
Hawkins, Richard A Kim, Donghee
Najmabadi, Feridoon McCormack, Charles E. Peterson, Darryl R. Rasgado-Flores, Hèctor
Dr. Bradbury's research interests lie in the area of regulation of membrane protein trafficking in polarized epithelia. We have used the chloride channel protein CFTR as a paradigm for the cAMP dependent regulation of apical membrane endocytic events. We are interested in the protein-protein interactions between adaptins, clathrin, and CFTR involved in the endocytic internalization of CFTR. Interest also lies in the cAMP-dependent protein kinase (PKA) regulation of CFTR channel activity and trafficking and its mediation by binding of PKA to subcellular anchor proteins (AKAPs).
Recent Publications
Bradbury, N.A., and Bridges R.J. (1994) Vesicular trafficking and solute transporter insertion into membranes. Topic Review Am. J. Physiol: Cell Physiol. 267: C1-C24. (Invited Review Article) Bradbury N.A. (1999). Role of intracellular CFTR in acidification. Physiol. Rev. 79: S175-S191 (Invited Review Article) Bradbury, N.A., Clark, J.A.,Watkins, S.C., Widnell, C., Smith, H.S., and Bridges, R.J. (1999). Characterization of the internalization pathways of the cystic fibrosis transmembrane conductance regulator (CFTR). Am. J. Physiol. 276: L659-L668 Weixel, K. and Bradbury, N.A. (1999). The carboxyl terminus of CFTR binds the endocytic adaptor complex AP-2. J. Biol. Chem. 275: 3655-3660. Singh, A.K., Schultz, B.D., Katzenellenbogen, J.A., Price, E.M., Bridges, R.J. and Bradbury, N.A. (2000). Estrogen inhibition of CFTR-mediated chloride secretion. J. Pharm. Exp. Ther. 295: 195-204. Sun, F., Hug, M., Bradbury, N.A. and Frizzell, R.A. (2000). E3KARP mediates the association of ezin and PKA with CFTR in airway cells. J. Biol. Chem. 275: 29539-29546. Weixel, K.M. and Bradbury, N.A. (2001). Endocytic adaptor complexes bind the C-terminal domain of CFTR. Pflugers Arch. – Eur. J. Physiol. 443(Suppl. 1): S70-S74. Bradbury, N.A. (2001). cAMP signaling cascades and CFTR: is there more to learn? Pflugers Arch. Eur. J. Physiol. 443(Suppl. 1): S85-S91. Weixel, K.M. and Bradbury, N.A. (2001). µ2 binding directs the cystic fibrosis transmembrane conductance regulator to the clathrin-mediated endocytic pathway. J. Biol. Chem. 276: 46251-46259. Silvis, M.R., Picciano, J.A., Bertrand, C., Bridges, R.J. and Bradbury, N.A. (2002). N287Y CFTR is a novel clinical mutation enhancing clathrin-mediated endocytosis. J. Clin. Invest. (Submitted). Oztan, A., Silvis, M., Weisz, O.A., Bradbury, N.A., Hsu, S-C., Goldenring, J.R., Yeamon, C., and Apodaca, G. (2007). Exocyst requirement for endocytic traffic directed toward the apical pole of polarized MDCK cells. Mol. Biol. Cell 18 3978-3992.