The Microscopy and Imaging Facility (MIF) is a University-wide facility that exists to advance university research productivity. Access is available to university researchers on a cost-recovery basis after their successful completion of certification training. The facility became fully operational in July, 2001, and is directed by Letia Peterson.
Equipment Available in the Microscopy and Imaging Facility
•Upright Olympus Fluoview300 Confocal Microscope with three laser lines (visible spectrum) equipped with Stereo Investigator software for confocal stereology.
•Inverted Olympus Fluoview300 Confocal Microscope with three laser lines (visible spectrum) equipped with Stereo Investigator software for confocal stereology.
•Olympus BH2 upright microscope with fluorescence equipped with Stereo Investigator software for brightfield and flourescence video-based stereology.
•Leica Laser Microdissection system
•Dedicated sterile Leica Cryostat for producing thin sections for laser microdissection.
•Bio-Rad iCycler real-time PCR system for use with laser microdissection.
Coming in 2008
MIF Training
A training and certification course is required in order to use the Microscopy and Imaging Facility. This is a comprehensive and intensive course based upon user courses taught at the Salk Institute and in Europe over the last ten years. It consists of three days of lecture followed by a fourth day of practical instruction at the microscopes. The goal of the course is to prepare users to correctly and safely operate the equipment while helping them to obtain the best possible data.
Fees for Usage
Equipment
Hourly Rate
Upright Confocal
$15.00
Offline computer station
$5.00
Cryostat
Bio-Rad iCycler real-time PCR
$20.00 per run
Laser Capture Microdissection
Brightfield Microscope
$10.00
MIF Contact Information
Letia Peterson Director, Microscopy and Imaging Facility Room: 1.374 Phone: 847-578-8661 Fax: 847-578-8545 Email: letia.peterson@rosalindfranklin.edu
Daniel A. Peterson, Ph.D. Scientific Advisor Room: 1.381B Phone: 847-578-3411 Fax: 847-578-8545 Email: daniel.peterson@rosalindfranklin.edu
Emily Reisenbigler Senior Research Specialist - Laser Microdissection Room: 1.374 Phone: 847-578-3506 Fax: 847-578-8545 Email: emily.reisenbigler@rosalindfranklin.edu
Scott Surridge Research Specialist - Microscopy Room: 1.378 Phone: 847-578-8640 Fax: 847-578-8545 Email: scott.surridge@rosalindfranklin.edu
Carol Galioto Senior Research Assistant - Billing Room: 1.374 Phone: 847-578-3520 Fax: 847-578-8545 Email: carol.galioto@rosalindfranklin.edu