Center for Stem Cell and Regenerative Medicine

Mission


 

To conduct basic medical research that advances knowledge of stem cell biology and tissue regeneration leading to the development of therapeutic strategies.



Dentate granule cell layer of an adult rat. GFAP-positive stem cells lie underneath the granule cell layer and generate new dentate granule neurons throughout life. Mature neurons are blue (NeuN), astrocytes are green (GFAP), and the nuclei of all cells are labeled red.  (Peterson Lab)

 


Areas of Research

 

Center Investigators

Daniel A. Peterson, Ph.D. (Director)
Professor and Vice-Chairman of Neuroscience                                   
The Chicago Medical School
daniel.peterson@rosalindfranklin.edu
Phone: 847.578.3411

Stephanie C. Wu, D.P.M
Associate Professor of Surgery
Director, C.L.E.A.R
Associate Dean for Research
Scholl College of Podiatric Medicine

Robert Marr, Ph.D.  
Assistant Professor of Neuroscience  
The Chicago Medical School
Beth Stutzmann, Ph.D.
Associate Professor of Neuroscience
The Chicago Medical School

Iris Seitz, M.D., Ph.D.
Assistant Professor of Surgery
The Chicago Medical School
Advocate Lutheran General Hospital 
Loren Schechter, M.D.
Associate Professor of Surgery
The Chicago Medical School
Advocate Lutheran General Hospital
 

Affiliated Investigators and Collaborators

 
Dorothy Kozlowski, Ph.D.
Associate Professor of Department of Biological Sciences
Chair of Department of Health Sciences
DePaul University

María Castelló, Ph.D.
Associate Scientist
Instituto de Investigaciones Clemente Estable
Universidad de la República,Uruguay

Tong Chuan He, M.D., Ph.D.
Associate Professor of Surgery 
University of Chicago

Rui Olivera, Ph.D.
Professor in Behavioural Biology
Instituto Gulbenkian de Ciência
Lisbon, Portugal

              

Resources

Confocal Stereology Research Laboratory


To analyze and quantify experimental outcomes of stem cell therapies, it is necessary to utilize the latest advances in imaging technology combined with rigorous quantitative histology by stereology.  To advance this analysis, the Center has established a dedicated research laboratory to develop automated, high-throughput stereological analysis. To achieve this sampling, the laboratory has an Olympus DSU confocal microscope for unattended high-throughput confocal stereology. There is a second Olympus microscope equipped for unattended high-throughput stereological sampling of brightfield material.  These systems are supported by an off-line analysis workstation.  

Viral Vector Research Laboratory


Delivery of functional or reporter transgenes to cells in vitro and in vivo is an important tool for the discovery of stem cell regulation and contribution to repair.  The Center has available resources for the preparation of retroviral and lentiviral vectors to support this research.

Stem Cell Culture Laboratory
  Center investigators have access to mammalian cell culture facilities separately dedicated to human and rodent cell culture.  Work conducted here includes both the isolation and characterization of adult tissue-derived stem cells and the generation of patient specific induced pluripotent cells (iPCs).
 
Laser Microdissection and Gene Expression Laboratory

 
Stem cells in tissue are rare and reside in particular environmental niches that make it difficult to assess gene expression without contamination from surrounding tissue.  To identify and collect spatially restricted samples in tissue, the Center has a Leica Laser microdissection system and equipment for downstream analysis of gene expression by quantitative PCR.  This resource supports studies identifying stem cells in tissue and their contribution to tissue repair. 
 

Flow Cytometry Facility
Flow cytometry is available to Center investigators through an excellent core facility directed by Bob Dickinson, operated under the supervision of Prof. Bala Chandran, Chairman of the Department of Microbiology and Immunology  More information is available here.


Working Groups

The goal of working groups is to enable investigators with common research interests and goals to work together toward the development of specific research collaborations and grant applications

TBI-PTSD Working Group
Core Members:  Dorothy Kozlowski, Dan Peterson, Janice Urban, Beth Stutzmann, Bob Marr

Meetings: February 14, 2013
March 8, 2013

Tissue Repair Working Group
Core Members:  Dan Peterson, Stephanie Wu, Loren Schechter, Iris Seitz, Bob Marr

Meetings: TBD

     

Life in Discovery
3333 Green Bay Road, North Chicago, Il 60064-3095 • 847-578-3000