Below are lists of reagents and experimental protocols used in laboratories of faculty within the School of Graduate and Postdoctoral Studies.  This resource is intended to identify experimental material as well as expertise within SGPS to facilitate individual and collaborate research by graduate students, postdoctoral fellows and faculty.

 Reagents

• Dr. Hongkyun Kim, 10/12/12
• Dr. Tony West, 10/12/12
• Dr. Joe DiMario, 10/12/12
• Dr. Gulam Waris, 10/18/12

     DNA Vectors and Expression Constructs

• DiMario Lab, Vectors, 10/12/12
• DiMario Lab, Signaling Proteins, 10/12/12
• DiMario Lab, Transcriptional Factors, 10/12/12

     Primers

• Waris Lab, 10/18/12

Techniques

     Cell Isolation

• Peripheral Blood Mononuclear Cells - Dr. Chandran

     Labeling

• Alexa546 Labeling (KcsA) - Dr. Sackin
• Tb Labeling (KcsA) - Dr. Sackin

• RNA Extraction and QRTPCR - Dr. Marr
• RNA Extraction and QRTPCR - Dr. Chandran

     DNA Analysis

• Electromobility Shift Assay - Dr. Chandran
• Chromatin Immunoprecipitation - Dr. DiMario
• Dual Luciferase Assay - Dr. DiMario
• Electromobility Shift Assay - Dr. DiMario

     Virology

• Small Scale Virus Prep - Dr. Marr
• Lentiviral GFP Titration - Dr. Marr
• Lentivirus Preparation - Dr. Marr

     Histochemistry and Immunohistochemistry

• Immunofluorescence Assay, B Cells - Dr. Chandran
• Immunofluorescence Assay, Adherent Cells - Dr. Chandran
• Detection of B-gal in Cells - Dr. DiMario
• Detection of B-gal in Cell Extracts - Dr. DiMario
• Whole Mount Staining - Dr. DiMario

     Immunoprecipitation

• IP and co-IP - Dr. Chandran
• Immunoprecipitation - Dr. DiMario

     Protein Expression and Purification

• Insect Cells - Dr. Chandran

     Cell Fractionation

• Lipid Raft Extraction - Dr. Chandran
• Nuclear Protein Extraction 1 - Dr. DiMario
• Nuclear Protein Extraction 2 - Dr. DiMario