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Neil Bradbury, PhD

Professor

Dr. Neil Bradbury received his PhD in medical biochemistry from the University of Wales in 1987. Prior to that, he received a bachelor’s degree (Hons) in biochemistry from the University of St. Andrews in Scotland.

Dr. Bradbury is a member of the American Physiological Society

Research Projects

Dr. Bradbury's research interests lie in the area of regulation of membrane protein trafficking in polarized epithelia. We have used the chloride channel protein CFTR as a paradigm for the cAMP dependent regulation of apical membrane endocytic events. We are interested in the protein-protein interactions between adaptins, clathrin, and CFTR involved in the endocytic internalization of CFTR. Interest also lies in the cAMP-dependent protein kinase (PKA) regulation of CFTR channel activity and trafficking and its mediation by binding of PKA to subcellular anchor proteins (AKAPs).

Recent Publications

  • Bradbury, N.A., and Bridges R.J. (1994) Vesicular trafficking and solute transporter insertion into membranes. Topic Review Am. J. Physiol: Cell Physiol. 267: C1-C24. (Invited Review Article)
  • Bradbury N.A. (1999). Role of intracellular CFTR in acidification. Physiol. Rev. 79: S175-S191 (Invited Review Article)
  • Bradbury, N.A., Clark, J.A.,Watkins, S.C., Widnell, C., Smith, H.S., and Bridges, R.J. (1999). Characterization of the internalization pathways of the cystic fibrosis transmembrane conductance regulator (CFTR). Am. J. Physiol. 276: L659-L668
  • Weixel, K. and Bradbury, N.A. (1999). The carboxyl terminus of CFTR binds the endocytic adaptor complex AP-2. J. Biol. Chem. 275: 3655-3660.
  • Singh, A.K., Schultz, B.D., Katzenellenbogen, J.A., Price, E.M., Bridges, R.J. and Bradbury, N.A. (2000). Estrogen inhibition of CFTR-mediated chloride secretion. J. Pharm. Exp. Ther. 295: 195-204.
  • Sun, F., Hug, M., Bradbury, N.A. and Frizzell, R.A. (2000). E3KARP mediates the association of ezin and PKA with CFTR in airway cells. J. Biol. Chem. 275: 29539-29546.
  • Weixel, K.M. and Bradbury, N.A. (2001). Endocytic adaptor complexes bind the C-terminal domain of CFTR. Pflugers Arch. – Eur. J. Physiol. 443(Suppl. 1): S70-S74.
  • Bradbury, N.A. (2001). cAMP signaling cascades and CFTR: is there more to learn? Pflugers Arch. Eur. J. Physiol. 443(Suppl. 1): S85-S91.
  • Weixel, K.M. and Bradbury, N.A. (2001). µ2 binding directs the cystic fibrosis transmembrane conductance regulator to the clathrin-mediated endocytic pathway. J. Biol. Chem. 276: 46251-46259.
  • Silvis, M.R., Picciano, J.A., Bertrand, C., Bridges, R.J. and Bradbury, N.A. (2002). N287Y CFTR is a novel clinical mutation enhancing clathrin-mediated endocytosis. J. Clin. Invest. (Submitted).
  • Oztan, A., Silvis, M., Weisz, O.A., Bradbury, N.A., Hsu, S-C., Goldenring, J.R., Yeamon, C., and Apodaca, G. (2007). Exocyst requirement for endocytic traffic directed toward the apical pole of polarized MDCK cells. Mol. Biol. Cell 18 3978-3992.
  • Ameen, N., Silvis, M.R., and Bradbury, N.A. (2007) Endocytic trafficking of CFTR in health and disease. J. Cyst. Fibros. 6 1-14.
  • Abban, C.Y., Bradbury, N.A. and Meneses, P.I. (2008). HPV16 and BPV1 infection can be blocked by the dynamin inhibitor dynasore. American Journal of Therapeutics 15 304-311.
  • Silvis, M.R., Bertrand, C., Ameen, N., Golin-Bisello, F., Butterworth, M., Frizzell, R.A. and Bradbury, N.A. (2009). Rab11b regulates apical recycling of CFTR in polarized epithelial cells. Mol.Biol. Cell. 20 2337-2350.
  • Young, A., Abban, C.Y., Gentzsch, M., Menes, P.I., Bridges, R.J. and Bradbury N.A. (2009). Dynasore inhibits the removal of wild-type and mutant CFTR from the cell surface. Biochem. J. 421 377-385.