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Macromolecular X-ray and Computing X-ray Facility

The facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects based on X-ray crystallography, single particle cryo electron microscopy and molecular modeling. Rosalind Franklin University is a member of SER-CAT (www2.ser.aps.anl.gov) with regular access to synchrotron beamlines 22ID and 22BM at Advanced Photon Source.  CryoEM data is collected at National Facilities including S2C2 and NCCAT (https://nccat.nysbc.org/).  Linux computing includes a 15TB-4GPU workstation for the cryoEM data processing and several graphics workstations for the x-ray crystallography.  Multiple software suites are kept current by Structural Biology Grid (www.sbgrid.org). Modeling packages include Molecular Operating Environment (www.chemcomp.com) and SCHRODINGER (www.schrodinger.com). The facility have equipment as listed below.

X-ray Facility Fee Schedule

Maintenance Information:

Useful X-ray DataProcessing guides:
HKL-Manual
d*trekwalk through

RFUMS X-ray Facility Resources:

  • Rigaku RU-h2R 5.4 kW X-ray generator with copper rotating anode 
  • Osmic confocal “green” fine focusing mirrors 
  • R-axis IV++ imaging plate area detector 
  • CCD video system for crystal mounting 
  • MSC X-Stream 2000 cryocooling system 
  • Bruker AXS SMART 6000 CCD detector with 3-circle goniometer 
  • Oxford cryocooling system 
  • TPP Labtech Mosquito crystallization robot 
  • Korima UV crystal detection and visualization system 
  • Liquid nitrogen 
  • Storage liquid nitrogen dewars 
  • Dry-shipping dewars 
  • Cryotools and crystal handling tools 
  • Six large incubators with temperature control 
  • Temperature-controlled room for crystal growth and manipulations 

RFUMS CryoEM  Facility Resources:

  • Linux 15TB-4GPU workstation.
  • PELCO easiGlowTM Glow Discharge system.
  • EOL JEM-1230, 120kV Transmission Electron Microscope (TEM) equipped with a high-resolution Hamamatsu ORCA-HR CCD Camera (2624 x 2400 pixel) with AMT Imaging Software for negative stained images.  

Building:  BSB
Room:  L.141
Phone:  847-578-8474