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Neelam Sharma-Walia, PHD

Associate Professor

Dr. Walia received her Ph.D. in Experimental Medicine and Biotechnology from the Postgraduate Institute of Medical Education and Research, Chandigarh, India in 1998. She was appointed as Senior Demonstrator in the Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India from 1998-2000. She worked as postdoctoral fellow in the Northwestern University Medical School, Chicago in 2001 and in the Department of Microbiology, Molecular Genetics and Immunology at University of Kansas Medical Center from 2002-2005. She joined the Rosalind Franklin University of Medicine and Science in July, 2005 as a Research Assistant Professor and was promoted to Assistant Professor in the Department of Microbiology and Immunology in July 2011.

Research

Our studies focus on arachidonic acid (AA) pathway in Kaposi’s sarcoma associated herpes virus (KSHV) pathogenesis. Our previous studies reported the upregulation of prostaglandin pathway’s major stress angiogenic protein cyclooxygenase-2 (COX-2) and its lipid metabolite prostaglandin E2 (PGE2) in the endothelial cells strictly displaying viral latency and Body-cavity B-cell lymphoma (BCBL) cells carrying multicopy viral genome. Our studies showed that KSHV induced COX-2/PGE2 play important role in KSHV pathogenesis related events like inflammation, invasion, angiogenesis and cell survival. Using a variety of cutting edge technologies, we are currently deciphering the role of prostaglandin pathway in KSHV episome maintenance and chromatin remodeling. Our research also focuses on the identification of the cellular and viral factors involved in the stability of COX-2 and continuous regulated secretion of PGE2 in KSHV positive lymphoma cells.

Peer-Reviewed Publications

  1. Chandrasekharan JA, Huang XM, Hwang A, Sharma-Walia N*. Altering the anti-inflammatory lipoxin microenvironment: a new insight into KSHV pathogenesis. J. Virol. 2016 Sep 28. pii: JVI.01491-16.
  2. Chandrasekharan JA, Marginean A, Sharma-Walia N*.  An insight into the role of arachidonic acid derived lipid mediators in virus associated pathogenesis and malignancies. Prostaglandins Other Lipid Mediat. 2016 Jul 20. pii: S1098-8823(16)30082-X. doi: 10.1016/j.prostaglandins.2016.07.009
  3. Goswami S, Sharma-Walia N. Crosstalk between osteoprotegerin (OPG), fatty acid synthase (FASN) and, cycloxygenase-2 (COX-2) in breast cancer: implications in carcinogenesis. Oncotarget. 2016 Jun 6. doi: 10.18632/oncotarget.9835. 
  4. Goswami S and Sharma-Walia N. Osteoprotegerin rich tumor microenvironment: implications in breast cancer. Oncotarget. doi: 10.18632/oncotarget.8658. April 8 2016.
  5. Goswami S and Sharma-Walia N. Osteoprotegerin secreted by inflammatory and invasive breast cancer cells induces aneuploidy, cell proliferation and angiogenesis. BMC Cancer. 2015 Nov 25;15(1):935. doi: 10.1186/s12885-015-1837-1.
  6. Chandran K#, Goswami S, Sharma-Walia N#. Implications of a peroxisome proliferator-activated receptor alpha (PPARα) ligand clofibrate in breast cancer. Oncotarget. 2015 Nov 26. doi: 10.18632/oncotarget.6402. # Both authors contributed equally to the work.
  7. Chandrasekharan JA, Sharma-Walia N. Lipoxins: nature's way to resolve inflammation. J Inflamm Res. 2015 Sep 30;8:181-92. doi: 10.2147/JIR.S90380. eCollection 2015.
  8. Marginean A#, Sharma-Walia N#. Lipoxins exert antiangiogenic and anti-inflammatory effects on Kaposi's sarcoma cells. Translational research : the journal of laboratory and clinical medicine. 2015. Epub 2015/03/31 (Featured New Investigator series, published as corresponding author). # Both authors contributed equally to the work.
  9. Valiya Veettil M, Bottero V, Dutta D, Bandyopadhyay C, Gjyishi O, Shrama-Walia N, Dutta S, and Chandran B. Glutamate secretion and mGluR1 expression during Kaposi’s sarcoma associated herpesvirus infection promotes cell proliferation. PLoS Pathog. 2014 Oct 9; 10(10):e1004389. doi: 10.1371/journal.ppat.1004389. eCollection 2014 Oct.
  10. Sharma-Walia, N, Chandran K, Patel K, Veettil MV, and Marginean A. The KSHV induced 5-lipoxygenase-leukotriene B4 (5LO/LTB4) cascade plays key roles in KSHV latency, monocyte recruitment and lipogenesis. Journal of Virology, 2014; 85: 1980-1993 (published as corresponding author).
  11. George-Paul A, Chandran B, Sharma-Walia N. COX-2-PGE2-EP receptor inflammatory axis: A key player in KSHV associated malignancies. Translational Research, 2013; 162(2):77-92. doi: 10.1016/j.trsl.2013.03.004. (published as corresponding author).
  12. George-Paul A, Chandran B, Sharma-Walia N. Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines. Translational Research, 2013; 161(6):447-68. doi: 10.1016/j.trsl.2013.02.008 (Featured New Investigator series, (published as corresponding author).
  13. Sharma-Walia N, Patel K, Chandran K, Marginean A, Bottero V, Kerur N, and George-Paul A COX-2/PGE2: molecular ambassadors of Kaposi’s sarcoma associated herpes virus (KSHV) oncoprotein-v-FLIP [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012, Mar31st-Apr4th Chicago, Illinois (IL), Abstract nr-LB-67.
  14. Sharma-Walia N, Patel K, Chandran K, Marginean A, Bottero V, Kerur N, and George-Paul A. COX-2/PGE2: molecular ambassadors of Kaposi’s sarcoma-associated herpes virus oncoprotein-v-FLIP. Oncogenesis, 2012, 1, e5; doi:10.1038/oncsis.2012.5 (published as corresponding author).
  15. Sadagopan S, Veettil MV, Chakraborty S, Sharma-Walia N, Paudel N, Bottero V, and Chandran B. Angiogenin functionally interacts with p53 and regulates p53-mediated apoptosis and cell survival. Oncogene. 2012 Jan 23. doi: 10.1038/onc.2011.648.
  16. George-Paul A, Sharma-Walia N and Chandran B. Targeting COX-2/KSHV latency and LANA-1/p53 links by nimesulide: A novel anti-viral/inflammatory/cancer strategy against Body cavity B cell lymphoma. PloS ONE, 2011. 6: e24379 (published as corresponding author).
  17. Kerur N, Veettil MV, Sharma-Walia N, Bottero V, Sadagopan S and Chandran B. IFI 16 acts as a nuclear danger sensor and induces the inflammasome in response to Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Infection. Cell Host Microbe, 19:363-375, 2011.
  18. Bottero V, Kerur N, Sadagopan, S, Patel K, Sharma-Walia, N. and Chandran B. TGFβ Activating Kinase 1 (TAK1) phosphorylation and polyubiquitination is necessary for KSHV G protein-coupled receptor (vGPCR) activation of NF-ĸB. J. Virol.85, 1980-1993, 2011.
  19. Sharma-Walia N, George-Paul A, Patel K, Chandran K, Ahmad W and Chandran B. NFAT and CREB regulate Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Induced Cyclooxygenase-2 (COX-2). J. Virol. 84, 12733-12753, 2010. (published as corresponding author
  20. Kerur N, Veettil, MV, Sharma-Walia N, Sadagopan, S, Bottero V, George-Paul A, Chandran B. Characterization of Entry and Infection of Monocytic THP-1 cells by Kaposi's Sarcoma-Associated Herpesvirus: Role of Heparan Sulfate, DC-SIGN, Integrins and Signaling. Virology, 406, 103-116. 2010
  21. George-Paul A, Sharma-Walia N, Kerur N, White C and Chandran B. Piracy of PGE2/EP receptor mediated signaling by Kaposi's sarcoma associated herpes virus (KSHV/HHV-8) for latency gene expression: Strategy of a successful pathogen. Cancer Res. 70, 3697-3708, 2010
  22. Sharma-Walia N, George-Paul A, Bottero V, Sadagopan S, Veettil MV, Kerur N and Chandran B.  Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Induced COX-2: A Key Factor in Latency, Inflammation, Angiogenesis, Cell Survival and Invasion. PloS Path.  6, P. e1000777, 2010 (published as corresponding author

    *This work was quoted as “must read” article by faculty of 1000.
  23. Bottero V, Sharma-Walia N, Kerur N, George-Paul A, Sadagopan S, Cannon M and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV) G Protein-Coupled Receptor (vGPCR) Activates the ORF50 Lytic Switch Promoter: A Potential Positive Feedback Loop for Sustained ORF50 Gene Expression. Virology, 392,34-51, 2009
  24. Raghu H, Sharma-Walia N, Veettil MV, Sadagopan S and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus Utilizes an Actin Polymerization-Dependant Macropinocytic Pathway To Enter Human Dermal Microvascular Endothelial and Human Umbilical Vein Endothelial Cells. J. Virol. 83, 4895-4911, 2009
  25. Sadagopan S, Sharma-Walia N, Veettil MV, Bottero V, Levine R, Vart RJ and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Upregulates Angiogenin During Infection of Human Dermal Microvascular Endothelial Cells Which Induces 45SrRNA Synthesis, Anti-apoptosis, Cell Proliferation, Migration and Angiogenesis. J. Virol. 83,3342-3364,2009
  26. Veettil MV, Sadagopan S, Sharma-Walia N, Wang FZ,  Raghu H, Varga L and Chandran B. Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) forms a Multi-Molecular Complex of Integrins (αVβ5, αVβ3 and α3β1) and CD98-xCT During Infection of Human Dermal Microvascular Endothelial (HMVEC-d) Cells and CD98-xCT is Essential for Post-Entry Stage of Infection. J. Virol. 82,12126-12144, 2008
  27. Sivakumar R, Sharma-Walia, N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R  and Chandran B. Kaposi’s sarcoma Associated Herpes Virus Induces Sustained Levels of Vascular Endothelial Growth Factors A and C Early During in vitro Infection of Human Microvascular Dermal Endothelial Cells:  Biological Implications.  J. Virol. 82,1759-1776, 2008
  28. Raghu H, Sharma-Walia N, Valiya VM, Sadagopan S, Caballero A, Sivakumar R, Varga L, Bottero V and Chandran B. Lipid Rafts of Primary Endothelial Cells are Essential for Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Induced PI3-K and RhoA-GTPases Critical for Microtubule Dynamics and Nuclear Delivery of Viral DNA but Dispensable for Binding and Entry. J. Virol. 81,7941-7959, 2007
  29. Sadagopan S, Sharma-Walia N, Veettil MV, Raghu H, Sivakumar R and Chandran B. Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Induces Sustained NF-kB Activation During de novo Infection of Primary Human Dermal Microvascular Endothelial Cells that is Essential for Viral Gene Expression. J. Virol. 81, 3949-3968, 2007
  30. Veettil MV, Sharma-Walia N, Sadagopan S, Raghu H, Ramu S, Naranatt PP and Chandran B. RhoA-GTPase Facilitates Entry of Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) into the Adherent Target cells in a Src Dependent Manner.J. Virol. 80, 11432-11446, 2006
  31. Sharma-Walia N, Raghu H, Sadagopan S, Ramu S, Veettil, MV, Naranatt PP, Smith M.M.  and Chandran B. Cyclooxygenase-2 Induced by Kaposi's Sarcoma-Associated Herpes Virus Early during In Vitro Infection of Target Cells Plays a Role in the Maintenance of Latent Viral Gene Expression. J. Virol. 80, 6534-6552, 2006
  32. Krishnan HH, Sharma-Walia N, Streblow DN, Naranatt PP and Chandran B. Focal Adhesion Kinase (FAK) is Critical for Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Entry into the Target Cells. J. Virol. 80,1167-1180, 2006
  33. Krishnan HH, Sharma-Walia N,  Zeng L, Smith, MS and Chandran B. Envelope glycoprotein gB of Kaposi's Sarcoma-Associated Herpes Virus (KSHV) is essential for egress from infected cells. J. Virol. 79,10952-10967, 2005
  34. Sharma-Walia N, Krishnan HH, Naranatt PP and Chandran B. KSHV/HHV-8 Envelope Glycoprotein gpK8.1A induced ERK Early During Infection is Essential for the Latent and Lytic gene Expression and for the Establishment of Latent Infection.J. Virol.79,10308-10329, 2005

Book Chapters

  1. Everly D and Sharma-Walia N., Sadagopan, S. and Chandran, B. Herpesviruses and Cancer. In:  Editor; E.  Springer Sciences Business Media) In Press.  Dec.2011).
  2. 2. Chandran B  Sharma-Walia N. KSHV entry and infection of target cells. In: DNA tumor viruses. Springer Sciences Business Media; 2009.p.583-609.