David H. Harrison, Ph.D.;
BMB secondary appointment
Director
Henry Symersky, Ph.D.
Facility Manager
Building: BSB
Room: L.141
Phone: 847-578-8474
The Department of Biochemistry & Molecular Biology has modern equipment providing the resources necessary for contemporary research in Protein Chemistry and Molecular Biology. Besides the standard equipment, the department has several core facilities.
X-ray Facility
The Macromolecular X-ray and Computing facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects that utilize X-ray crystallography and molecular modeling. Core instrumentation for crystallization consists of the robot Mosquito from TPP Labtech, and the Korima UV fluorescence visualization system. In-house diffraction experiments are performed on an R-axis area detector or a Bruker CCD detector, using the high-brilliance Rigaku generator, a rotating copper anode, and Oxford mirrors. Cryogenic cooling is provided by the Rigaku X-stream or the Oxford Cryosystem. Synchrotron diffraction data is collected at APS Argonne National Laboratory near Chicago. Rosalind Franklin University is a member of SER-CAT (www.ser.aps.anl.gov) with regular access to APS beamlines 22ID and 22BM. Computing is based on a 6TB Linux server and several NFS-connected client computers with 3D graphics. Multiple software suites are kept up to date by Structural Biology Grid (www.sbgrid.org). Modeling packages include Molecular Operating Environment (www.chemcomp.com) and SCHRODINGER (www.schrodinger.com).
X-ray Facility Fee Schedule (Effective 03/01/13)
The Core Investigators
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INVESTIGATOR NAME
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PROJECT
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David H. T. Harrison, Ph.D., Director BMB secondary appointment
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Structural Enzymology, Methylglyoxal Synthase, HMG-CoA Synthase, and Hydroxyphenyl pyruvate
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Henry Symersky, Ph.D. Facilities Manager
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Departmental projects utilizing macromolecular x-ray crystallography
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Jun-Yong Choe, Ph.D., BMB
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Glucose transporters
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Carl Correll, Ph.D., BMB
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RNA Chaperones
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Marc Glucksman, Ph.D., BMB
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Structural neurobiology of processing enzymes
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Ronald Kaplan, Ph.D., BMB and Vice Dean of Research (CMS)
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Mitochondrial transport proteins
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Min Lu, Ph.D., BMB
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Multidrug and Toxin Extrusion (MATE) transporters
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David Mueller, Ph.D., BMB
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Yeast ATP synthase and cystic fibrosis transmembrane conductance regulator
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Kyoung Joon Oh, Ph.D.,BMB
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Program cell death pathways
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Eric Walters, Ph.D., BMB
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HIV-1 protease/non-peptidyl inhibitors
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Dmitri Boudko, Ph.D., PB
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Transporters |
Independent User Time:
Users from other institutions (NIH funded or other) are encouragedto apply for time to use the instrumentation (contact Dr.Harrison for details).
Maintenance Information:
Useful X-ray DataProcessing guides:
HKL-Manual
d*trekwalk through
FOR NIH GRANTS
Description of X-Ray Facility:
Resources: RFUMS has an X-ray facility that contains the following equipment: A Rigaku RU-H3R 5.4 kW X-ray rotating anode generator with Osmic confocal “green” fine focusing mirrors that produce high brilliance, collimated, monochromatic Cu-Ka X-rays; Image data can be collected on either a R-axis IV++ imaging plate area detector with CCD video system for crystal mounting and an MSC X-Stream 2000 cryocooling system or a Bruker AXS SMART 6000 CCD detector with a 3-circle goniometer and an Oxford liquid N2 cryogenic cooling system. Additionally, RFUMS is a member of SER-CAT and is granted regularly scheduled access to the APS beamlines 22ID and 22BM at Argonne National Laboratory. Crystallization trials are assisted using a TPP Labtech Mosquito crystallization robot, six large temperature controlled incubators with temperature control, a temperature controlled room, and a Korima UV crystal detection and visualization system optimized for protein crystal detection.
RFUMS X-ray Facility Resources:
- Rigaku RU-H3R 5.4 kW X-ray generator with copper rotating anode
- Osmic confocal “green” fine focusing mirrors
- R-axis IV++ imaging plate area detector
- CCD video system for crystal mouting
- MSC X-Stream 2000 cryocooling system
- Bruker AXS SMART 6000 CCD detector with 3-circle goniometer
- Oxford cryocooling system
- TPP Labtech Mosquito crystallization robot
- Korima UV crystal detection and visualization system
- Liquid nitrogen
- Storage liquid nitrogen dewars
- Dry-shipping dewars
- Cryotools and crystal handling tools
- Six large incubators with temperature control
- Temperature-controlled room for crystal growth and manipulations
- Eight Linux workstations with 3D graphics that utilize the latest crystallographic and modeling software supported by SBGgrid (sbgrid.org/software)
- Regular access to APS synchrotron beamlines 22ID and 22BM