Macromolecular X-ray and Computing



David H. Harrison, Ph.D.;
BMB secondary appointment

Director

Henry Symersky, Ph.D.
Facility Manager

Building:  BSB
Room:  L.141
Phone:  847-578-8474

The Department of Biochemistry & Molecular Biology has modern equipment providing the resources necessary for contemporary research in Protein Chemistry and Molecular Biology. Besides the standard equipment, the department has several core facilities.

X-ray Facility

The Macromolecular X-ray and Computing facility is located at L141 of the main campus, 3333 Green Bay Road, North Chicago, IL 60064. It supports structural biology projects that utilize X-ray crystallography and molecular modeling. Core instrumentation for crystallization consists of the robot Mosquito from TPP Labtech, and the Korima UV fluorescence visualization system. In-house diffraction experiments are performed on an R-axis area detector or a Bruker CCD detector, using the high-brilliance Rigaku generator, a rotating copper anode, and Oxford mirrors. Cryogenic cooling is provided by the Rigaku X-stream or the Oxford Cryosystem. Synchrotron diffraction data is collected at APS Argonne National Laboratory near Chicago. Rosalind Franklin University is a member of SER-CAT (www.ser.aps.anl.gov) with regular access to APS beamlines 22ID and 22BM. Computing is based on a 6TB Linux server and several NFS-connected client computers with 3D graphics.  Multiple software suites are kept up to date by Structural Biology Grid (www.sbgrid.org). Modeling packages include Molecular Operating Environment (www.chemcomp.com) and SCHRODINGER (www.schrodinger.com).  

  

 

X-ray Facility Fee Schedule(Effective 02/01/2014)

The Core Investigators

INVESTIGATOR NAME

PROJECT

David H. T. Harrison, Ph.D., Director BMB secondary appointment

Structural Enzymology, Methylglyoxal Synthase, HMG-CoA Synthase, and Hydroxyphenyl pyruvate

Henry Symersky, Ph.D. Facilities Manager

Departmental projects utilizing macromolecular x-ray crystallography

Jun-Yong Choe, Ph.D., BMB

Glucose transporters

Carl Correll, Ph.D., BMB

RNA Chaperones

Marc Glucksman, Ph.D., BMB

Structural neurobiology of processing enzymes

Ronald Kaplan, Ph.D., BMB and Vice Dean of Research (CMS)

Mitochondrial transport proteins

Min Lu, Ph.D., BMB

Multidrug and Toxin Extrusion (MATE) transporters

David Mueller, Ph.D., BMB

Yeast ATP synthase and cystic fibrosis transmembrane conductance regulator

Kyoung Joon Oh, Ph.D.,BMB

Program cell death pathways

Eric Walters, Ph.D., BMB

HIV-1 protease/non-peptidyl inhibitors

Dmitri Boudko, Ph.D., PB


Transporters

Independent User Time:
Users from other institutions (NIH funded or other) are encouraged to apply for time to use the instrumentation (contact Dr. Harrison for details).

Maintenance Information:

Useful X-ray DataProcessing guides:
HKL-Manual
d*trekwalk through

FOR NIH GRANTS

Description of X-Ray Facility:

Resources:  RFUMS has an X-ray facility that contains the following equipment: A Rigaku RU-H3R 5.4 kW X-ray rotating anode generator with Osmic confocal “green” fine focusing mirrors that produce high brilliance, collimated, monochromatic Cu-Ka X-rays; Image data can be collected on either a R-axis IV++ imaging plate area detector with CCD video system for crystal mounting and an MSC X-Stream 2000 cryocooling system or a Bruker AXS SMART 6000 CCD detector with a 3-circle goniometer and an Oxford liquid N2 cryogenic cooling system. Additionally, RFUMS is a member of SER-CAT and is granted regularly scheduled access to the APS beamlines 22ID and 22BM at Argonne National Laboratory. Crystallization trials are assisted using a TPP Labtech Mosquito crystallization robot, six large temperature controlled incubators with temperature control, a temperature controlled room, and a Korima UV crystal detection and visualization system optimized for protein crystal detection.

RFUMS X-ray Facility Resources:

  • Rigaku RU-H3R 5.4 kW X-ray generator with copper rotating anode
  • Osmic confocal “green” fine focusing mirrors
  • R-axis IV++ imaging plate area detector
  • CCD video system for crystal mounting
  • MSC X-Stream 2000 cryocooling system
  • Bruker AXS SMART 6000 CCD detector with 3-circle goniometer
  • Oxford cryocooling system
  • TPP Labtech Mosquito crystallization robot
  • Korima UV crystal detection and visualization system
  • Liquid nitrogen
  • Storage liquid nitrogen dewars
  • Dry-shipping dewars
  • Cryotools and crystal handling tools
  • Six large incubators with temperature control
  • Temperature-controlled room for crystal growth and manipulations
  • Eight Linux workstations with 3D graphics that utilize the latest crystallographic and modeling software supported by SBGgrid (sbgrid.org/software)
  • Regular access to APS synchrotron beamlines 22ID and 22BM
Life in Discovery
Department Home
Students and Postdoctorals
Deborah L. Faiman and Dr. Laurence R. Meyerson Biochemistry and Molecular Biology Student Travel Award
3333 Green Bay Road, North Chicago, Il 60064-3095 • 847-578-3000