Virginie Bottero, Ph.D.

Research Assistant Professor

Room 2.419
Telephone (847) 578-8835
Biography

Dr. Bottero received her Ph.D. in Molecular and Cellular Aspects of Biology from the University of Nice-Sophia Antipolis, France in 2002. She worked as a postdoctoral fellow at the Salk Institute for Biological Studies, La Jolla, CA from 2002 to 2006. She joined Rosalind Franklin University of Medicine and Science in July 2006 as a Senior Research Associate.

RESEARCH

Human herpes virus-8 (HHV-8, KSHV) is a recently identified herpes virus implicated in the etiology of Kaposi’s sarcoma and primary effusion lymphoma in AIDS patients and immune-suppressed transplant patients. Our current research goal is to understand how and why the virus hijacks host signaling pathways and activate some cellular transcription factors. Such results are expected to have an important positive impact because the identified component will lead to the development of new therapeutic approaches to control KSHV infection and related malignancies.

PEER-REVIEWED PUBLICATION

1.      Bottero V, Rossi F, Samson M, Mari M, Hofman P and Peyron JF. IkB-a, the NF-kB inhibitory subunit, interacts with ANT, the mitochondrial ATP/ADP translocator. J Biol Chem. 2001;276(24):21317-24.

2.       Bottero V, Busuttil V, Loubat A, Magné N., Fischel JL, Milano G and Peyron JF. The topoisomerase poisons SN38 and doxorubicin activate NF-kB through the IKK complexCancer Research 2001, 61, 7785-7791.

3.       Cursio R, Mari B, Louis K, Rostagno P, Saint-Paul MC, Giudicelli J, Bottero V, Anglard P, Yiotakis A, Dive V, Gugenheim J and Auberger P. Rat Liver Injury Following Normothermic Ischemia is prevented by a Phosphinic Matrix Metalloproteinase Inhibitor. FASEB J. 2002;16(1):93-5.

4.      Busuttil V, Bottero V, Frelin C, Imbert V, RicciJE, Auberger P, PeyronJF. The blockade NF-kB activation in Jurkat leukemic T cells converts the survival agent and tumor promoter PMA into an apoptotic effectorOncogene, 2002, vol. 21, no. 20 pp. 3213-3224.

5.      Tergaonkar V, Bottero V, Ikawa M, Li Q, Verma IM. IkB kinase-independent IkBa degradation pathway: functional NF-kB activity and implications for cancer therapy. Mol Cell Biol. 2003;23(22):8070-83.

6.      Bottero V, Imbert V, Frelin C, Formento JL, Peyron JF. Monitoring NF-kB Transactivation Potential Via Real-Time PCR Quantification of IkB-a Gene Expression. Mol Diagn. 2003;7(3):187-94.

7.      Ducut JL, Bottero V, Young DB, Shevchenko A, Mercurio F, Verma IM. ELKS, a regulatory subunit of IkB kinase complex required for NF-kB activation. Science. 2004;304(5679):1963-7.

8.       Li Q, Lu Q, Bottero V, Estepa G, Morrison L, Mercurio F and Verma IM. Enhanced NF-kB activation and cellular function in macrophages lacking IkB kinase 1 (IKK1). Proc Natl Acad Sci U S A. 2005; 102(35) :12425-30

9.      Magne N, Toillon RA, Bottero V, Didelot C, Houtte PV, Gerard JP, Peyron JF. NF-kB modulation and ionizing radiation: mechanisms and future directions for cancer treatment. Cancer Lett. 2006;231(2):158-68.

10.  Woods NB, Bottero V, Verma IM. Therapeutic gene for XSCID gene therapy trial results in T-cell lymphoma in mice, Nature.2006;440(7088):1123.

11.  Muotri AR, Bottero V, Tergaonkar V, Correa RG. UV-mediated NF-kappaB activation is abolished in deficient XPC/D primary fibroblasts. Cell Cycle. 2006 May;5(10):1085-9.

12.  Ghosh S, Tergaonkar V, Rothlin CV, Correa RG, Bottero V, Bist P, Verma IM, Hunter T. Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kB activation and cell survival. Cancer Cell. 2006;10(3):215-26.

13.  Sadagopan S, Sharma-Walia N, Veettil MV, Raghu H, Sivakumar R, Bottero V, Chandran B. Kaposi's sarcoma-associated herpesvirus induces sustained NF-kappaB activation during de novo infection of primary human dermal microvascular endothelial cells that is essential for viral gene expression. J Virol. 2007;81(8):3949-68.

14.  Raghu H, Sharma-Walia N, Veettil MV, Sadagopan S, Caballero A, Sivakumar R, Varga L, Bottero V, Chandran B. Lipid Rafts of Primary Endothelial Cells Are Essential for Kaposi's Sarcoma-Associated Herpesvirus (KSHV/HHV-8) Induced PI3-K and RhoA-GTPases Critical for Microtubule Dynamics and Nuclear Delivery of Viral DNA but Dispensable for Binding and Entry. J Virol. 2007;81(15):7941-59.

15.  Frelin C, Imbert V, Bottero V, Gonthier N, Samraj AK, Schulze-Osthoff K, AubergerP, CourtoisG, Peyron JF. Inhibition of the NF-kB survival pathway via caspase-dependent cleavage of the IKK complex scaffold protein and NF-kB essential modulator NEMO. Cell Death Differ .2008;15(1):152-60.

16.  Sivakumar R, Sharma-Walia N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R, Chandran B. Kaposi's sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implicationsJ Virol. 2008;82(4):1759-76.

17.  Sadagopan S, Sharma-Walia N, Veettil MV, Bottero V, Levine R, Vart RJ, Chandran B. Kaposi's Sarcoma Associated Herpes Virus (KSHV/HHV-8) Upregulates Angiogenin During Infection of Human Dermal Microvascular Endothelial Cells Which Induces 45SrRNA Synthesis, Anti-apoptosis, Cell Proliferation, Migration and Angiogenesis.J Virol. 2009;83(7):3342-64.

18.  Xia Y, Padre RC, De Mendoza TH, Bottero V, Tergaonkar VB, Verma IM. Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP. Proc Natl Acad Sci U S A. 2009;106(8):2629-34.

19.  Bottero V, Sharma-Walia N, Kerur N, Paul AG, Sadagopan S, Cannon M, Chandran B. Kaposi Sarcoma-associated herpes virus (KSHV) G protein-coupled receptor (vGPCR) activates the ORF50 lytic switch promoter: A potential positive feedback loop for sustained ORF50 gene expression. Virology. 2009 ;392(1):34-51.

20.  Sharma-Walia N, Bottero V#, Paul AG#, Sadagopan S, Veettil MV, Kerur N, Chandran B. Kaposi’s sarcoma associated herpes virus (KSHV) induced cyclooxygenase (COX)-2: A key factor in latency, inflammation, angiogenesis, cell survival and invasion. PLoS Pathog. 2010 Feb 12;6(2):e1000777.

21.  Kerur N, Veettil MV, Sharma-Walia N, Sadagopan S, Bottero V, Paul AG, Chandran B. Characterization of entry and infection of monocytic THP-1 cells by Kaposi's sarcoma associated herpesvirus (KSHV): role of heparan sulfate, DC-SIGN, integrins and signaling. Virology. 2010 Oct 10;406(1):103-16.

22.  Bottero V*, Patel K, Sharma-Walia N, Sadagopan S, Chandran B. TGFb Activating Kinase 1 (Tak1) is involved in NF-kB activation and tumorigenesis induced by Kaposi Sarcoma-associated herpes virus (KSHV) G protein-coupled receptor (vGPCR). J Virol. 2011 Mar;85(5):1980-93.

23.  Sadagopan S, Veettil MV, Paudel N, Bottero V, Chandran B. Kaposi`s sarcoma-associated herpesvirus (KSHV) induced angiogenin play roles in latency via PLCg pathway and blocking angiogenin inhibits lantent gene expression and induces lytic cycle. J Virol. 2011 Mar;85(6):2666-85.

24.  Kerur N, Veettil MV, Sharma-Walia N, Bottero V, Sadagopan S, Otageri P, Chandran B. IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus. Cell Host Microbe. 2011 May 19;9(5):363-75.

25.  Sadagopan S, Veettil MV, Chakraborty S, Sharma-Walia N, Paudel N, Bottero V, Chandran B. Angiogenin functionally interacts with p53 and regulates p53-mediated apoptosis and cell survival. Oncogene. 2012 Jan 23. doi:10.1038/onc.2011.648. 

26.  Sharma-Walia, N, Patel. K,  Chandran K, Bottero V, Kerur N, Paul AG, and Chandran B. Role of v FLIP in COX-2 induction and KSHV latency. Oncogenesis. (2012) 1, e5; doi:10.1038/oncsis.2012.5

27.  Chakraborty S, ValiyaVeettil M, Bottero V, Chandran B. Kaposi’s sarcoma-associated herpesvirus interacts with EphrinA2 receptor to amplify signaling essential for productive infection. Proc. Natl. Acad. Sci. USA. 2012 May 8;109(19):E1163-72.

28.  Bottero V *, Chakraborty S, Chandran B. Reactive Oxygen Species Are Induced by Kaposi's Sarcoma-Associated Herpesvirus Early during Primary Infection of Endothelial Cells To Promote Virus Entry. J Virol. 2013 Feb;87(3):1733-49.

29.  Singh VV#, Kerur N#, Bottero V#, Dutta S, Chakraborty S, Ansari MA, Paudel N, Chikoti L, Chandran B. Kaposi's Sarcoma-Associated Herpesvirus Latency in Endothelial and B cells Activates Interferon Gamma-Inducible Protein 16 (IFI16) Mediated Inflammasomes. J Virol. 2013 Apr;87(8):4417-31.

# Both authors contributed equally.

* corresponding author

REVIEWS

1.      Bottero V, Withoff S, Verma IM. NF-kB and the regulation of hematopoiesis. Review article. Cell Death Differ. 2006;13(5):785-97.

2.      Bottero V, Busuttil V, Frelin C, Imbert V and Peyron JF. Les facteurs de transcription NFkappaB: Des mécanismes moléculaires d'activation à la régulation de la survie et de la prolifération cellulaires. Regard sur la Biochimie 2001:31-39. (Review article in French).

UPDATED: 06.25.2013

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