The mammalian cell nucleus is a highly organized organelle, which reflects the complex regulation of its multiple activities. The nucleus contains an ever-growing number of specialized compartments, many of which contain disease gene products such as spinal muscular atrophy, Xeroderma Pigmentosum, Werner syndrome, amongst others.
One of the most important questions in modern cell biology is how gene expression is organized in the cell nucleus. Recent discoveries suggest that the nuclear interior is highly organized and nuclear processes may depend on the dynamics of nuclear components and compartments. Fundamental questions concerning the understanding of the dynamic organization of gene expression have not been answered: How are nuclear compartments maintained in the membrane-less interior of the nucleus? How are transcription and RNA processing complexes regulated within the nucleus? What are the molecular mechanisms that coordinate the functions of these complexes in the highly dynamic nuclear environment? I would like to address these aspects of gene expression and nuclear function using in vivo
imaging combined with molecular biology/biochemical approaches.