Dr. Jaiswal received his M.Sc. in Biochemistry from Devi Ahilaya University, Indore India in 2003, and his Ph.D. degree in Biochemistry from Jiwaji University, Gwalior, India, in 2010. Dr. Jaiswal's postdoctoral experience includes positions as Postdoctoral fellow and then as Postdoc Research Associate Reproductive and Tumor Immunology laboratory at the Rosalind Franklin University of Medicine, North Chicago, USA, between June 2009- June 2014. He was appointed as Research Assistant Professor in the Reproductive and Tumor Immunology laboratory at the RFUMS in July 2014 and he is under the mentorship of Dr. Kenneth D. Beaman.
Research in the Reproductive and Tumor Immunology laboratory on five related themes:
1. Investigating the role of innate immune response in preterm labor.
Preterm birth is considered to be the most important cause of neonatal morbidity and mortality not due to congenital anomalies in the developed world. Our group recent studies show that TRL ligands induce preterm labor in the murine model via simultaneous activation of apoptosis and inflammatory processes. This hyper-inflammation in preterm labor is directly with defective autophagy.
2. Leukocyte regulates the blastocyst implantation and placentation.
Antigen presenting cells (APCs) are essential for initiating immune tolerance for pregnancy and uterine immune responses to infection. Uterine macrophages are master regulators of tolerance to paternal alloantigen, through generating regulatory T cells that prevent maternal immune rejection of the conceptus. Our current projects show that infiltration of both M1 and M2 macrophage is critical for successful implantation and placentation. Additionally, these cells have tissue remodeling functions and communicate with epithelial cells and stromal cells to impact endometrial receptivity for blastocyst implantation.
3. Immune regulation of successful implantation, placental development, spermatogenesis and reproductive outcome.
Cytokines initiating either from the uterine epithelium or paternal side act to regulate the development of the embryo and leads to successful implantation. Our studies show an important role for a2V-ATPase in promoting optimal blastocyst development, implantation and placental development, and in programming paternal immune response for offspring. In humans, a2V-ATPase promotes healthy sperm production and application of this discovery to improve implantation success in human IVF is now the subject of clinical trials.
4. Semen signaling in the female reproductive tract.
Our study provides new insights into the role of capacitation in facilitating pregnancy through the regulation of immune process, which is characterized by the expression of a2V-ATPase and inflammatory cytokines in capacitated sperm. Given the critical role that the release of a2V-ATPase subcomponent a2NTD (N-terminus domain) from the capacitated sperm and its involvement in the expression of inflammatory cytokines; there is strong evidence that it is an essential molecule for onset of pregnancy. Following fertilization of the ova, the embryo at the maternal interface takes over the regulation of the local immune response by expressing a2V. In the post-mating period, the inflammatory phase is confirmed by the recruitment of M1 macrophages in the early pregnant uterus which is the reflective event of the upregulation a2V-ATPase and Mcp1 expression. This study also provides evidence that the exposure of both sperm and seminal plasma is crucial for facilitating pregnancy through upregulation of a2V-ATPase and panel of genes that confers uterine receptivity near the window of implantation.
5. Role of a2 isoform of Vacuolar ATPase in tumor growth.
Our recent studies establish the role of a2V-ATPase in modulating a macrophage phenotype towards TAMs through the action of a2NTD, suggesting it to be a potential therapeutic target in cancer.
1. Katara G.K., Kulshrestha A., Jaiswal M.K., Gilman-Sachs, A., and Beaman, K.D. (2015) - Inhibition of vacuolar ATPase subunit in tumor cells delays tumor growth by decreasing the essential macrophage population in the tumor microenvironment. Oncogene (Accepted).
2. Gilman-Sachs A., Tikoo, A., Akman-Anderson, L., Jaiswal, M.K., Ntrivalas, E., Beaman, K.D. (2015) - Expression and role of a2 vacuolar-ATPase (a2V) in trafficking of human neutrophil granules and exocytosis. Journal of Leukocyte Biology. (Accepted).
3. Agrawal V.*, Jaiswal M.K.*, Mallers T.M., Gilman-Sachs A, Beaman KD, Hirsch E. (2015) – Altered autophagic flux enhances inflammatory responses during inflammation-induced preterm labor. Scientific Reports (Accepted). (*These authors contributed equally to work).
4. Kulshrestha, A., Katara G.K, Ibrahim, S., Pamarthy, S., Jaiswal M.K., Gilman-Sachs, A., and Beaman, K.D. (2014)- Vacuolar ATPase 'a2' isoform exhibits distinct cell surface accumulation and modulates matrix metalloproteinase activity in ovarian cancer. Oncotarget (Accepted).
5. Agrawal V., Jaiswal, M.K., Ilievski V, Jilling T, Beaman, K.D. Hirsch E. (2014) - Platelet Activating Factor (PAF): A Role in Preterm Delivery and an Essential Interaction with Toll-Like Receptor (TLR) Signaling. Biology of Reproduction 2014 Sep 24. pii: biolreprod.113.116012.
6. Beaman K.D., Jaiswal M.K., Svetlana Dambaeva, Gilman-Sachs A., (2014). Future Directions of Clinical Laboratory Evaluation of Pregnancy. Cellular and Molecular Immunology. doi: 10.1038/cmi.2014.62.
7. Jaiswal M.K., Katara G.K., Mallers T., Chaouat G., Gilman-Sachs A., Beaman K.D. (2014). Vacuolar- ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis. Journal of Leukocyte Biology. 96(2):337-47.
8. Katara G.K., Jaiswal M.K., Kulshrestha A., Kolli, B., Gilman-Sachs, A., and Beaman, K.D. (2013). Tumor associated vacuolar ATPase subunit promotes tumorigenic characteristics in macrophages. Oncogene. doi:10.1038/onc.2013.532.
9. Jaiswal M.K.*, Agrawal V.*, Mallers T., Gilman-Sachs, A., Hirsch E., and Beaman, K.D. (2013). Regulation of Apoptosis and innate stimuli during inflammation induced preterm labor. Journal of Immunology. 191(11):5702-13. (*These authors contributed equally to work).
10. Agrawal V, Jaiswal M.K., Jaiswal Y.K. (2013). LPS-Induced Implantation Failure: One of the Causes of Female Infertility. Med J Obstet Gynecol 1(3): 1014.
11. Ota K., Jaiswal M.K., Ramu S., Jeyendran R., Kwak-Kim J., Gilman-Sachs A., Beaman K.D. (2013) . Expression of a2 Vacuolar ATPase in spermatozoa 1 is associated with semen quality and chemokine-cytokine profiles in infertile men. Plos One 8(7): e70470. doi:10.1371/journal.pone.0070470.12.
12. Chueh F.U., Cronk R.J., Alsuwaidan A.N., Mallers T., Jaiswal M.K., Beaman K.D. & Yu C.L. (2013). Mouse LSTRA leukemia as a model of human NKT and highly aggressive lymphoid malignancies. Leukemia & Lymphoma. doi:10.3109/10428194.2013.810740.
13. Jaiswal M.K.*, Agrawal V.*, Jaiswal Y.K. (2013). Lipopolysaccharide drives alternation of Heat Shock Proteins and induces failure of blastocyst implantation in mouse. Biology of Reproduction 88(6) 162, 1-12. (*These authors contributed equally to work).
14. Agrawal V.*, Jaiswal, M.K.*, Jaiswal Y.K. (2013). Lipopolysaccharide induced modulation in the expression of progesterone receptor and estradiol receptor leads to early pregnancy loss in mouse. Zygote. 21(4):337-344. (*These authors contributed equally to work).
15. Beaman, K. D., Ntrivalas, E., Mallers, T., Jaiswal, M.K., Kwak-Kim, J. and Gilman-Sachs, A. (2012). Immune etiology of recurrent pregnancy loss and its diagnosis. American Journal of Reproductive Immunology. 67 (4) 319-325.
16. Jaiswal M.K., Mallers T., Kwong, C., Chaouat G., Gilman-Sachs A., Beaman K. (2012). Abortive-prone mating drives alteration of systemic a2 Vacuolar-ATPase expression in Mice. American Journal of Reproductive Immunology. 67(5) 421–433.
17. Agrawal V.*, Jaiswal, M.K.*, Jaiswal Y.K. (2012). Gonadal and nongonadal FSHR and LHR dysfunction during gram-negative bacterial infection induced failure of mice blastocyst implantation. Journal of Assisted Reproduction and Genetics. 29(2) 163-173 (*These authors contributed equally to work).
18. Jaiswal M.K., Larsen B., Mallers T., Kwak-Kim J., Chaouat G., Gilman-Sachs A., Beaman K. 2012 A2V-ATPase Upregulation during Capacitation: A possible link to establishment of an inflammatory response during preimplantation period of pregnancy. Reproduction. 143(5):713-25.
19. Jaiswal M.K., Gilman-Sachs A., Chaouat G., Beaman K. (2011) - Placental ATPase Expression is a Link Between Multiple Causes of Spontaneous Abortion. Biology of Reproduction 2011 85( 3) 626-634.
20. Agrawal V.*, Jaiswal M.K.*, Jaiswal Y.K. (2011) - Lipopolysaccharide induces alterations in ovaries and serum level of progesterone and 17β-estradiol in mouse. Fertility and Sterility 95(4) 1471-1474. (*These authors contributed equally to work).
21. Jaiswal, M.K., Agrawal V., Jaiswal Y.K. (2011) - Effect of Polymyxin B on Gram-negative Bacterial Infection mouse model. J Turkish-German Gynecol Assoc 2011; 12: 64-70.
22. Agrawal V., Jaiswal M.K., Chaturvedi M.M., Tiwari D.C., Jaiswal Y.K. (2009) – Lipopolysaccharide Alters the Vaginal Electrical Resistance in Cycling and Pregnant mice. American Journal of Reproductive Immunology 61(2) 158-166.
23. Jaiswal, Y.K., Jaiswal, M.K., Agrawal V., Chaturvedi, M.M. (2009) - Bacterial Endotoxin (LPS) Induced DNA damage in Preimplanting Embryonic and Uterine Cells Inhibit Implantation. Fertility and sterility 91(5 Suppl): 2095-2103.
24. Jaiswal, Y.K., Jaiswal, M.K., Agrawal Varkha, Chaturvedi, M.M., Deb, K. (2007) - Maternal Gram-negative bacterial infection induced apoptosis of the implanting blastocyst. Journal of Turkish and German Gynecology Association 8 (2): 190-193.