| Ph.D. Yale University, 1964
|| Mailing address:
3333 Green Bay Road
North Chicago, IL 60064
Dr. Kemp received his B.A. in Chemistry from the College of Wooster in 1959 and his Ph.D. in Biochemistry from Yale University in 1964. Following post-doctoral studies at the University of Washington, he was appointed assistant professor at the Medical College of Wisconsin, where from 1968 to 1973, he was an Established Investigator of The American Heart Association. In 1971, Dr. Kemp was a Fulbright Fellow to The Southern University of Chile. Dr. Kemp was appointed Professor in 1975 and moved to the Finch University of Health Sciences/The Chicago Medical School as chairman of Biochemistry in 1976, a position he held until 1989. Dr. Kemp has served on the research committee of the Leukemia Research Foundation and was a member of a National Institutes of Health Study Section.
Metabolic control mechanisms; structure/function studies of allosteric enzymes.
Dr. Kemp's interest is in cellular regulatory mechanisms with particular emphasis on the structure and properties of allosteric enzymes. The laboratory focuses on phosphofructokinase, the key regulatory enzyme of carbohydrate metabolism in virtually all organisms. This enzyme has complex regulatory properties and displays tremendous regulatory diversity throughout the biosphere.
- Allosteric properties of mammalian phosphofructokinase and the nature of allosteric binding sites are being studied by a combination of recombinant DNA and chemical techniques including site-directed mutagenesis, affinity modification methodology, protein sequence analysis, equilibrium binding studies, polymeric state analysis and kinetic studies.
- The mechanism of catalysis is under investigation with particular emphasis on the PPi-dependent enzyme from parasitic protozoa.
Ghosh S., Chan J.M., Lea C.R., Meints G.A., Lewis J.C., Tovian Z.S., Flessner R.M., Loftus T.C., Bruchhaus I., Kendrick H., Croft S.L., Kemp R.G., Kobayashi S., Nozaki T., Oldfield E. (2004) Effects of bisphosphonates on the growth of Entamoeba histolytica and Plasmodium species in vitro and in vivo. J. Med. Chem. 47, 175-187. [pubmed]
Sotgia F., Bonuccelli G., Minetti C., Woodman S.E., Capozza F., Kemp R.G., Scherer P.E., Lisanti M.P. (2003) Phosphofructokinase muscle-specific isoform requires caveolin-3 expression for plasma membrane recruitment and caveolar targeting: implications for the pathogenesis of caveolin-related muscle diseases. Am. J. Pathol. 163, 2619-2634. [pubmed]
Kemp R.G., Gunasekera D. (2002) Evolution of the allosteric ligand sites of mammalian phosphofructo-1-kinase. Biochemistry 41, 9426-9430. [pubmed]
Chang S.H., Kemp R.G. (2002) Role of Ser530, Arg292, and His662 in the allosteric behavior of rabbit muscle phosphofructokinase. Biochem. Biophys. Res. Commun. 290, 670-675. [pubmed]
Wang X., Kemp R.G. (2001) Reaction path of phosphofructo-1-kinase is altered by mutagenesis and alternative substrates. Biochemistry 40, 3938-3942. [pubmed]
Chi A.S., Deng Z., Albach R.A., Kemp R.G. (2001) The two phosphofructokinase gene products of Entamoeba histolytica. J. Biol. Chem. 276, 19974-19981. [pubmed]
Gunasekera D., Kemp R.G. (2000) Genomic organization, 5'flanking region and tissue-specific expression of mouse phosphofructokinase C gene. Gene 260, 103-112. [pubmed]
Chi A. and Kemp R.G. (2000) The primordial high energy compound: ATP or inorganic pyrophosphate? J. Biol. Chem. 275, 35677-35679. [pubmed]
Deng Z., Wang X., Kemp R.G. (2000) Site-directed mutagenesis of the fructose 6-phosphate binding site of the pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica. Arch. Biochem Biophys 380, 56-62. [pubmed]
Wang, X., and Kemp, R.G. (1999) Identification of residues of Escherichia coli phosphofructokinase that contribute to nucleotide binding and specificity. Biochemistry 38, 4313-4318. [pubmed]
Gunasekera, D., and Kemp, R.G. (1999) Cloning, sequencing , expression, and purification of the C isozyme of mouse phosphofructokinase. Prot. Express. Purif. 16, 448-453. [pubmed]
Deng, Z., Roberts, D., Wang, X., and Kemp, R.G. (1999) Expression, characterization and crystallization of the pyrophosphate-dependent phosphofructo-1-kinase of Borrelia burgdorferi. Arch. Biochem. Biophys. 371, 326-331. [pubmed]
Li, Y., Rivera, D., Ru, W., Gunasekera, D., and Kemp, R. G. (1999) Identification of allosteric sites in rabbit phosphofructo-1-kinase. Biochemistry 38, 16407-16412. [pubmed]