Kenneth E. Neet, Ph.D.

Professor and Associate Dean of Research
Chicago Medical School
Biochemistry and Molecular Biology
Room: 3.132
Building: BSB
Phone: 847.578.3220
Fax: 847.578.3240
Recent Publications

For e-Print (PDF file) contact Kenneth Neet

Neet, K. E., Mahapatra, S. and Mehta, H. (2011) Selectivity of Cell Signaling in the Neuronal Response Based on NGF Mutations and Peptidomimetics in the Treatment of Alzheimers Disease in “Alzheimer’s Disease Pathogenesis-Core Concepts, Shifting Paradigms, and Therapeutic Targets” (ISBN 979-953-307-022-2), Intech, pp. 243-272. (

Bai, Y., Sivori, D., Woo, S.B., Neet, K.E, Lerner SF, Saragovi HU. (2011) During glaucoma, alpha2-macroglobulin accumulates in aqueous humor and binds to nerve growth factor, neutralizing neuroprotection. Invest Ophthalmol Vis Sci. 2011 Jul 15;52(8):5260-5265. PMID: 21642630

Aboulkassim, T., Tong, X.-K., Tse, Y. C., Wong, T.-P., Woo, S. B., Neet, K. E., Brahimi, F., Hamel, E., & Saragovi, H. U. (2011). Ligand-dependent TrkA activity in brain differently affects spatial learning and long-term memory. Molecular Pharmacology, 80, 498-508. PMID: 21616921

Bai, Y., Dergham, P., Nedev, H., Xu, J., Galan, A., Rivera, J.C., ZhiHua, S., Mehta, H., Woo, S. B., Sarunic, M.V., Neet, K.E., and Saragovi, H.U. (2010) Chronic and acute models of retinal neurodegeneration TrkA activity is neuroprotective whereas p75NTR activity is neurotoxic through a paracrine mechanism. J. Biol. Chem. 285, 39392-39400. PMID: 20943663

Rogers, M.L., Bailey, S., Matusica, D., Nicholson, I., Muyderman, H., Pagadala, P.C., Neet, K.E., Zola, H., Macardle, P., and Rush, R.A. (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-Sortlin complex. J. Neuroimmunol. 226, 93–103. PMID: 20547427

Mahapatra S, Mehta H, Woo SB, Neet KE., Identification of critical residues within the conserved and specificity patches of nerve growth factor leading to survival or differentiation. J Biol Chem. (2009) 284, 33600-13. medline

Neet, K.E. (2009) Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects. In Contemporary Enzyme Kinetics and Mechanism, 3rd Ed. D. L. Purich, ed. Academic Press [updated & reprinted from 1995 article]

Masoudi R, Ioannou MS, Coughlin MD, Pagadala P, Neet KE, Clewes O, Allen SJ, Dawbarn D, Fahnestock M., Biological activity of nerve growth factor precursor is dependent upon relative levels of its receptors. J Biol Chem. (2009) 284:18424-33. medline

Neet KE and Thinakaran G., Thematic mini-review series on the molecular basis of Alzheimer disease. J Biol Chem. (2008) 283, 29613-4. medline

Boutilier J, Ceni C, Pagdala PC, Forgie A, Neet KE, Barker PA., Proneurotrophins require endocytosis and intracellular proteolysis to induce TrkA activation. J Biol Chem. (2008) 283:12709-16. medline


Ivanisevic, L., Zheng, W., Woo, S. B., Neet, K. E., and Saragovi, H. U. TrkA receptor "hot spots" for binding of NT-3 as a heterologous ligand. J Biol Chem. (2007) 282, 16754-16763. medline

Pagadala, P. C., Dvorak, L. A., and Neet, K. E. Construction of a mutated pro nerve growth factor resistant to degradation and suitable for biophysical and cellular utilization. Proc. Natl. Acad. Sci, USA (2006) 103, 17939-17943. medline

Vaghefi, H. and Neet, K.E. Deacetylation of p53 after nerve growth factor treatment in PC12 cells as a post-translational modification mechanism of neurotrophin induced tumor suppressor activation. Oncogene (2004) 23, 8078-8087. medline

Vaghefi H, Hughes A.L., Neet K.E. Nerve Growth Factor Withdrawal-mediated Apoptosis in Naive and Differentiated PC12 Cells through p53/Caspase-3-dependent and -independent Pathways. J Biol Chem. (2004) 279, 15604-15614. medline

Lad, S.P., Neet, K.E. and Mufson, E.J. Nerve growth factor: structure, function, and therapeutic implications in Alzheimer's disease. Curr Drug Target CNS Neurol Disord. (2003) 2, 315-334. (Review)

Lad, S. P. and Neet, K.E. Activation of the MAP Kinase Pathway through p75NTR: A Common Mechanism for the Neurotrophin Family" J. Neurosci. Res. (2003) 73, 614-626.

Lad, S. P., Peterson, D. A., Bradshaw, R.A., and Neet, K.E. Individual and Combined Effects of TrkA and p75NTR Nerve Growth Factor (NGF) Receptors: A Role for the High Affinity Receptor Site. J. Biol. Chem. (2003) 278, 24808-24817.

Pappas, T. C., Decorti, F., Macdonald, N. J., Neet, K. E., Taglialatela, G. Tumour necrosis factor-alpha vs. growth factor deprivation-promoted cell death: different receptor requirements for mediating nerve growth factor-promoted rescue. Aging Cell (2003) 2, 83-92.

Neet KE, Lee JC.,
Biophysical characterization of proteins in the post-genomic era of proteomics.
Mol Cell Proteomics. 2002 Jun;1(6):415-20. Review.

Ross GM, Shamovsky IL, Woo SB, Post JI, Vrkljan PN, Lawrance G, Solc M,
Dostaler SM, Neet KE, Riopelle RJ.,
The binding of zinc and copper ions to nerve growth factor is differentially affected by pH: implications for cerebral acidosis.
J Neurochem. 2001 Aug;78(3):515-23.

Neet KE, Campenot RB.
Receptor binding, internalization, and retrograde transport of neurotrophic factors.
Cell Mol Life Sci. 2001 Jul;58(8):1021-35. Review.
Abstract and Figures

Hughes AL, Messineo-Jones D, Lad SP, Neet KE.
Distinction between differentiation, cell cycle, and apoptosis signals in PC12 cells by the nerve growth factor mutant delta9/13, which is selective for the p75 neurotrophin receptor.
J Neurosci Res. 2001 Jan 1;63(1):10-9.
Abstract and Figures

Hughes AL, Gollapudi L, Sladek TL, Neet KE.
Mediation of nerve growth factor-driven cell cycle arrest in PC12 cells by p53. Simultaneous differentiation and proliferation subsequent to p53 functional inactivation.
J Biol Chem. 2000 Dec 1;275(48):37829-37.
Abstract and some Figures

Saragovi HU, Zheng W, Maliartchouk S, DiGugliemo GM, Mawal YR, Kamen A,
Woo SB, Cuello AC, Debeir T, Neet KE.
A TrkA-selective, fast internalizing nerve growth factor-antibody complex induces trophic but not neuritogenic signals.
J Biol Chem. 1998 Dec 25;273(52):34933-40.
Abstract, Figures, and Table

Neet KE.
Enzyme catalytic power minireview series.
J Biol Chem. 1998 Oct 2;273(40):25527-8. Review.
No abstract available, but if you are within the university, the link will take you to the full article.

Woo SB, Whalen C, Neet KE.
Characterization of the recombinant extracellular domain of the neurotrophin receptor TrkA and its interaction with nerve growth factor (NGF).
Protein Sci. 1998 Apr;7(4):1006-16.
Abstract and Figures

Gollapudi L, Neet KE.
Different mechanisms for inhibition of cell proliferation via cell cycle proteins in PC12 cells by nerve growth factor and staurosporine.
J Neurosci Res. 1997 Aug 15;49(4):461-74.
Abstract and Inhibition Pathway.

Woo SB, Neet KE.
Characterization of histidine residues essential for receptor binding and activity of nerve growth factor.
J Biol Chem. 1996 Oct 4;271(40):24433-41.
Abstract and Histidine Models

Guo M, Meyer SL, Kaur H, Gao JJ, Neet KE.
Mutational studies of conserved residues in the dimer interface of nerve growth factor.
Protein Sci. 1996 Mar;5(3):447-55.

Campbell XZ, Neet KE.
Hierarchical analysis of the nerve growth factor-dependent and nerve growth factor-independent differentiation signaling pathways in PC12 cells with protein kinase inhibitors.
J Neurosci Res. 1995 Oct 1;42(2):207-19.

Woo SB, Timm DE, Neet KE.
Alteration of NH2-terminal residues of nerve growth factor affects activity and Trk binding without affecting stability or conformation.
J Biol Chem. 1995 Mar 17;270(11):6278-85.

Neet KE.
Cooperativity in enzyme function: equilibrium and kinetic aspects.
Methods Enzymol. 1995;249:519-67. Review. No abstract available.

Neet KE, Timm DE.
Conformational stability of dimeric proteins: quantitative studies by equilibrium denaturation.
Protein Sci. 1994 Dec;3(12):2167-74. Review.

Timm DE, de Haseth PL, Neet KE.
Comparative equilibrium denaturation studies of the neurotrophins: nerve growth factor, brain-derived neurotrophic factor, neurotrophin 3, and neurotrophin 4/5.
Biochemistry. 1994 Apr 19;33(15):4667-76.

Timm DE, Ross AH, Neet KE.
Circular dichroism and crosslinking studies of the interaction between four neurotrophins and the extracellular domain of the low-affinity neurotrophin receptor.
Protein Sci. 1994 Mar;3(3):451-8.

Singh KR, Taylor LK, Campbell XZ, Fields AP, Neet KE.
A bryostatin-sensitive protein kinase C required for nerve growth factor activity.
Biochemistry. 1994 Jan 18;33(2):542-51.

Kasaian MT, Jacobberger JW, Neet KE.
Flow cytometric analysis of fluorescein-labeled nerve growth factor binding to A875 human melanoma cells.
Exp Cell Res. 1994 Jan;210(1):77-85.

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