Dr. Walia received her Ph.D. in Experimental Medicine and Biotechnology from the Postgraduate Institute of Medical Education and Research, Chandigarh, India in 1998. She was appointed as Senior Demonstrator in the Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India from 1998-2000. She worked as postdoctoral fellow in the Northwestern University Medical School, Chicago in 2001 and in the Department of Microbiology, Molecular Genetics and Immunology at University of Kansas Medical Center from 2002-2005. She joined the Rosalind Franklin University of Medicine and Science in July, 2005 as a Research Assistant Professor and was promoted to Assistant Professor in the Department of Microbiology and Immunology in July 2011.
Our studies focus on arachidonic acid (AA) pathway in Kaposi’s sarcoma associated herpes virus (KSHV) pathogenesis. Our previous studies reported the upregulation of prostaglandin pathway’s major stress angiogenic protein cyclooxygenase-2 (COX-2) and its lipid metabolite prostaglandin E2 (PGE2) in the endothelial cells strictly displaying viral latency and Body-cavity B-cell lymphoma (BCBL) cells carrying multicopy viral genome. Our studies showed that KSHV induced COX-2/PGE2 play important role in KSHV pathogenesis related events like inflammation, invasion, angiogenesis and cell survival. Using a variety of cutting edge technologies, we are currently deciphering the role of prostaglandin pathway in KSHV episome maintenance and chromatin remodeling. Our research also focuses on the identification of the cellular and viral factors involved in the stability of COX-2 and continuous regulated secretion of PGE2 in KSHV positive lymphoma cells.
1. Valiya Veettil M, Bottero V, Dutta D, Bandyopadhyay C, Gjyishi O, Shrama-Walia N, Dutta S, and Chandran B. Glutamate secretion and mGluR1 expression during Kaposi’s sarcoma associated herpesvirus infection promotes cell proliferation. PLoS Pathog. 2014 Oct 9; 10(10):e1004389. doi: 10.1371/journal.ppat.1004389. eCollection 2014 Oct.
2. Sharma-Walia, N, Chandran K, Patel K, Veettil MV, and Marginean A. The KSHV induced 5-lipoxygenase-leukotriene B4 (5LO/LTB4) cascade plays key roles in KSHV latency, monocyte recruitment and lipogenesis. Journal of Virology, 2014; 85: 1980-1993 (published as corresponding author).
3. George-Paul A, Chandran B, Sharma-Walia N. COX-2-PGE2-EP receptor inflammatory axis: A key player in KSHV associated malignancies. Translational Research, 2013; 162(2):77-92. doi: 10.1016/j.trsl.2013.03.004. (published as corresponding author).
4. George-Paul A, Chandran B, Sharma-Walia N. Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines. Translational Research, 2013; 161(6):447-68. doi: 10.1016/j.trsl.2013.02.008 (Featured New Investigator series, published as corresponding author).
5. Sharma-Walia N, Patel K, Chandran K, Marginean A, Bottero V, Kerur N, and George-Paul A COX-2/PGE2: molecular ambassadors of Kaposi’s sarcoma associated herpes virus (KSHV) oncoprotein-v-FLIP [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012, Mar31st-Apr4th Chicago, Illinois (IL), Abstract nr-LB-67.
6. Sharma-Walia N, Patel K, Chandran K, Marginean A, Bottero V, Kerur N, and George-Paul A. COX-2/PGE2: molecular ambassadors of Kaposi’s sarcoma-associated herpes virus oncoprotein-v-FLIP. Oncogenesis, 2012, 1, e5; doi:10.1038/oncsis.2012.5 (published as corresponding author).
7. Sadagopan S, Veettil MV, Chakraborty S, Sharma-Walia N, Paudel N, Bottero V, and Chandran B. Angiogenin functionally interacts with p53 and regulates p53-mediated apoptosis and cell survival. Oncogene. 2012 Jan 23. doi: 10.1038/onc.2011.648.
8. George-Paul A, Sharma-Walia N and Chandran B. Targeting COX-2/KSHV latency and LANA-1/p53 links by nimesulide: A novel anti-viral/inflammatory/cancer strategy against Body cavity B cell lymphoma. PloS ONE, 2011. 6: e24379 (published as corresponding author).
9. Kerur N, Veettil MV, Sharma-Walia N, Bottero V, Sadagopan S and Chandran B. IFI 16 acts as a nuclear danger sensor and induces the inflammasome in response to Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Infection. Cell Host Microbe, 19:363-375, 2011.
10. Bottero V, Kerur N, Sadagopan, S, Patel K, Sharma-Walia, N. and Chandran B. TGFβ Activating Kinase 1 (TAK1) phosphorylation and polyubiquitination is necessary for KSHV G protein-coupled receptor (vGPCR) activation of NF-ĸB. J. Virol.85, 1980-1993, 2011.
11. Sharma-Walia N, George-Paul A, Patel K, Chandran K, Ahmad W and Chandran B. NFAT and CREB regulate Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Induced Cyclooxygenase-2 (COX-2). J. Virol. 84, 12733-12753, 2010. (published as corresponding author)
12. Kerur N, Veettil, MV, Sharma-Walia N, Sadagopan, S, Bottero V, George-Paul A, Chandran B. Characterization of Entry and Infection of Monocytic THP-1 cells by Kaposi's Sarcoma-Associated Herpesvirus: Role of Heparan Sulfate, DC-SIGN, Integrins and Signaling. Virology, 406, 103-116. 2010
13. George-Paul A, Sharma-Walia N, Kerur N, White C and Chandran B. Piracy of PGE2/EP receptor mediated signaling by Kaposi's sarcoma associated herpes virus (KSHV/HHV-8) for latency gene expression: Strategy of a successful pathogen. Cancer Res. 70, 3697-3708, 2010
14. Sharma-Walia N, George-Paul A, Bottero V, Sadagopan S, Veettil MV, Kerur N and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV) Induced COX-2: A Key Factor in Latency, Inflammation, Angiogenesis, Cell Survival and Invasion. PloS Path. 6, P. e1000777, 2010 (published as corresponding author)
*This work was quoted as “must read” article by faculty of 1000.
15. Bottero V, Sharma-Walia N, Kerur N, George-Paul A, Sadagopan S, Cannon M and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV) G Protein-Coupled Receptor (vGPCR) Activates the ORF50 Lytic Switch Promoter: A Potential Positive Feedback Loop for Sustained ORF50 Gene Expression. Virology, 392,34-51, 2009
16. Raghu H, Sharma-Walia N, Veettil MV, Sadagopan S and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus Utilizes an Actin Polymerization-Dependant Macropinocytic Pathway To Enter Human Dermal Microvascular Endothelial and Human Umbilical Vein Endothelial Cells. J. Virol. 83, 4895-4911, 2009
17. Sadagopan S, Sharma-Walia N, Veettil MV, Bottero V, Levine R, Vart RJ and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Upregulates Angiogenin During Infection of Human Dermal Microvascular Endothelial Cells Which Induces 45SrRNA Synthesis, Anti-apoptosis, Cell Proliferation, Migration and Angiogenesis. J. Virol. 83,3342-3364,2009
18. Veettil MV, Sadagopan S, Sharma-Walia N, Wang FZ, Raghu H, Varga L and Chandran B. Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) forms a Multi-Molecular Complex of Integrins (αVβ5, αVβ3 and α3β1) and CD98-xCT During Infection of Human Dermal Microvascular Endothelial (HMVEC-d) Cells and CD98-xCT is Essential for Post-Entry Stage of Infection. J. Virol. 82,12126-12144, 2008
19. Sivakumar R, Sharma-Walia, N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R and Chandran B. Kaposi’s sarcoma Associated Herpes Virus Induces Sustained Levels of Vascular Endothelial Growth Factors A and C Early During in vitro Infection of Human Microvascular Dermal Endothelial Cells: Biological Implications. J. Virol. 82,1759-1776,2008
20. Raghu H, Sharma-Walia N, Valiya VM, Sadagopan S, Caballero A, Sivakumar R, Varga L, Bottero V and Chandran B. Lipid Rafts of Primary Endothelial Cells are Essential for Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Induced PI3-K and RhoA-GTPases Critical for Microtubule Dynamics and Nuclear Delivery of Viral DNA but Dispensable for Binding and Entry. J. Virol. 81,7941-7959, 2007
21. Sadagopan S, Sharma-Walia N, Veettil MV, Raghu H, Sivakumar R and Chandran B. Kaposi’s Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Induces Sustained NF-kB Activation During de novo Infection of Primary Human Dermal Microvascular Endothelial Cells that is Essential for Viral Gene Expression. J. Virol. 81, 3949-3968, 2007
22. Veettil MV, Sharma-Walia N, Sadagopan S, Raghu H, Ramu S, Naranatt PP and Chandran B. RhoA-GTPase Facilitates Entry of Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) into the Adherent Target cells in a Src Dependent Manner.J. Virol. 80, 11432-11446, 2006
23. Sharma-Walia N, Raghu H, Sadagopan S, Ramu S, Veettil, MV, Naranatt PP, Smith M.M. and Chandran B. Cyclooxygenase-2 Induced by Kaposi's Sarcoma-Associated Herpes Virus Early during In Vitro Infection of Target Cells Plays a Role in the Maintenance of Latent Viral Gene Expression. J. Virol. 80, 6534-6552, 2006
24. Krishnan HH, Sharma-Walia N, Streblow DN, Naranatt PP and Chandran B. Focal Adhesion Kinase (FAK) is Critical for Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Entry into the Target Cells. J. Virol. 80,1167-1180, 2006
25. Krishnan HH, Sharma-Walia N, Zeng L, Smith, MS and Chandran B. Envelope glycoprotein gB of Kaposi's Sarcoma-Associated Herpes Virus (KSHV) is essential for egress from infected cells. J. Virol. 79,10952-10967, 2005
26. Sharma-Walia N, Krishnan HH, Naranatt PP and Chandran B. KSHV/HHV-8 Envelope Glycoprotein gpK8.1A induced ERK Early During Infection is Essential for the Latent and Lytic gene Expression and for the Establishment of Latent Infection.J. Virol.79,10308-10329, 2005
27. Sharma-Walia N, Naranatt PP, Krishnan, HH, Zeng L and Chandran B. Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Envelope Glycoprotein gB Induces the Integrin Dependent Focal Adhesion Kinase-Src-PI-3K-RhoGTPase Signal Pathways and Cytoskeletal Rearrangements. J. Virol. 78,4207-4223, 2004
28. Akula SM, Naranatt PP, Sharma-Walia, N, Wang FZ, Fegley B and Chandran B Kaposi's Sarcoma-Associated Herpes Virus (KSHV/HHV-8) Infection of Human Fibroblast Cells Occurs Through Endocytosis. J. Virol. 77, 7978-7990, 2003
29. Wang FZ, Akula SM, Sharma-Walia N, Zeng L and Chandran B. Human Herpes Virus Envelope Glycoprotein gB Mediates Cell Adhesion via its RGD Sequence. J. Virol.77,3131-3147, 2003
30. Chugh S, Sharma-Walia N and Kanwar YS. Nephrotic Syndrome Induced Heterologous Phase Proteinuria in Aminopeptidase a Knock-out Mice. J. of the American Society of Neph. 12,674A, 2001
31. Kaushal S, Ghosh S, Sharma N, Sanyal SM and Majumdar S. Role of Phospholipid Transfer Protein in Rabbit Lung Development. Cell. and Mol. Life Sci. 58, 2098-2107, 2001
32. Grover V, Ghosh, S, Sharma N, Chakraborti A, Majumdar S and Ganguly NK Characterization of a Galactose Specific Adhesion of Aggregative Escherichia coli. Archives of Biochem. and Biophys. 390,109-118, 2001
33. Sharma, N, Desigan B, Sanyal SN, Majumdar S and Ganguly NK Effect of Antioxidant Vitamin E as a Protective Factor in Experimental Atherosclerosis in Rhesus Monkeys. Annals. of Nutr. and Metabol., 43,1-10, 1999
34. Sharma N, Desigan B, Ghosh S, Sanyal SN, Ganguly NK and Majumdar S. The Role of Oxidized HDL in Monocyte/Macrophage Function in the Pathogenesis of Atherosclerosis. Scand. J. of Clinical Lab. Invest. 59,215-226, 1999
1. Everly D and Sharma-Walia N., Sadagopan, S. and Chandran, B. Herpesviruses and Cancer. In: Editor; E. Springer Sciences Business Media) In Press. Dec.2011).
2. Chandran B Sharma-Walia N. KSHV entry and infection of target cells. In: DNA tumor viruses. Springer Sciences Business Media; 2009.p.583-609.
3. Dani HM, N Sharma and Harkirat. Anticarcinogenicity of flavonoids as studied by inhibition of lipid peroxidation microsomal degranulation and then interaction with Benza(a)pyrene metabolites. In: Eds. Ong. ASH and Packer, Birkhauser Verlag: Basel; 1992. p. 320-330